Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김정목 | - |
dc.date.accessioned | 2018-02-13T03:58:17Z | - |
dc.date.available | 2018-02-13T03:58:17Z | - |
dc.date.issued | 2011-09 | - |
dc.identifier.citation | SCANDINAVIAN JOURNAL OF IMMUNOLOGY, Vol.74, No.3 [2011], p253-263 | en_US |
dc.identifier.issn | 0300-9475 | - |
dc.identifier.uri | http://www.scopus.com/record/display.uri?eid=2-s2.0-79961100785&origin=inward&txGid=be120e7d26812839430946f3d3ca252b | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11754/37027 | - |
dc.description.abstract | DA-6034 is a synthetic derivative of eupatilin, a flavonoid with anti-inflammatory effects. The aim of this study was to investigate the effects of DA-6034 on the interactions between I kappa B kinase (IKK) and heat shock protein 90 (Hsp90), and activation of the nuclear factor-kappaB (NF-kappa B) signalling pathway in human gastric epithelial cells infected with Helicobacter pylori. MKN-45 gastric epithelial cell line was treated with DA-6034 and H. pylori. DA-6034 significantly inhibited NF-kappa B activation and upregulated the expressions of interleukin-8 (IL-8) and monocyte chemoattractant protein-1 in MKN-45 cells infected with H. pylori. However, DA-6034 did not influence activator protein-1 DNA binding activity in H. pylori-infected gastric epithelial cells. Pretreatment with DA-6034 attenuated the H. pylori-induced increase in IKK activity, and Hsp90 was associated with IKK-alpha and IKK-gamma in MKN-45 cells. Treatment with DA-6034 dissociated the Hsp90 and IKK-gamma complex in H. pylori-infected cells, leading to the inhibition of IL-8 expression. These results suggest that the eupatilin derivative 7-carboxymethyloxy-3',4',5-trimethoxy flavone has anti-inflammatory activity in gastric epithelial cells infected with H. pylori through the promotion of the dissociation of the IKK-gamma-Hsp90 complex and suppression of NF-kappa B signalling. | en_US |
dc.description.sponsorship | We thank Dr Martin F. Kagnoff and Dr Joseph A. DiDonato for providing the reporter gene plasmids, Dr Gang Min Hur for the HA-tagged IKK-α, IKK-β and IKK-γ constructs, Dr Ik-Sang Kim for the pMCP-1-luciferase plasmid and Dr Andreas von Knethen for the TAM-67 plasmid. This study was supported by a grant from the National Research Foundation (NRF) of Korea Grant funded by the Korean Government (MEST) (MRC Program No. 2010-0029507). | en_US |
dc.language.iso | en | en_US |
dc.publisher | WILEY-BLACKWELL, COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA | en_US |
dc.subject | BACTEROIDES-FRAGILIS ENTEROTOXIN | en_US |
dc.subject | CLOSTRIDIUM-DIFFICILE TOXIN | en_US |
dc.subject | PROTEIN-KINASE | en_US |
dc.subject | CHEMOATTRACTANT PROTEIN-1 | en_US |
dc.subject | VACUOLATING CYTOTOXIN | en_US |
dc.subject | CHEMOKINE EXPRESSION | en_US |
dc.subject | SIGNALING PATHWAY | en_US |
dc.subject | HSP90 COMPLEX | en_US |
dc.subject | IKK-GAMMA | en_US |
dc.subject | INFLAMMATION | en_US |
dc.title | A Mechanism for the Action of the Compound DA-6034 on NF-kappa B Pathway Activation in Helicobacter pylori-Infected Gastric Epithelial Cells | en_US |
dc.type | Article | en_US |
dc.relation.no | 3 | - |
dc.relation.volume | 74 | - |
dc.identifier.doi | 10.1111/j.1365-3083.2011.02577.x | - |
dc.relation.page | 253-263 | - |
dc.relation.journal | SCANDINAVIAN JOURNAL OF IMMUNOLOGY | - |
dc.contributor.googleauthor | Ko, SuHyuk | - |
dc.contributor.googleauthor | Yoo, Do-Young | - |
dc.contributor.googleauthor | Kim, Yeong-Jeon | - |
dc.contributor.googleauthor | Choi, Seul-Min | - |
dc.contributor.googleauthor | Kang, Kyungkoo | - |
dc.contributor.googleauthor | Kim, Hye-Young | - |
dc.contributor.googleauthor | Kim, Na-Young | - |
dc.contributor.googleauthor | Kim, Joo-Sung | - |
dc.contributor.googleauthor | Kim, Jung-Mogg | - |
dc.relation.code | 2011208508 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF MEDICINE[S] | - |
dc.sector.department | DEPARTMENT OF MEDICINE | - |
dc.identifier.pid | jungmogg | - |
dc.identifier.researcherID | 34771324000 | - |
dc.identifier.orcid | 0000-0002-6506-7519 | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.