Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이창호 | - |
dc.date.accessioned | 2018-02-12T08:22:41Z | - |
dc.date.available | 2018-02-12T08:22:41Z | - |
dc.date.issued | 2011-07 | - |
dc.identifier.citation | Journal of Cell Science, 2011, 124(13), P.2220-2230 | en_US |
dc.identifier.issn | 0021-9533 | - |
dc.identifier.uri | http://jcs.biologists.org/content/124/13/2220 | - |
dc.description.abstract | Recent evidence suggests that inflammation is involved in malignant progression of breast cancer. Sphingosine 1-phosphate (S1P), acting on the G-protein-coupled receptors, is known as a potent inflammatory mediator. In this study, the effect of the inflammatory lipid S1P on the regulation of invasive/migratory phenotypes of MCF10A human breast epithelial cells was investigated to elucidate a causal relationship between inflammation and the control of invasiveness of breast cells. We show that S1P causes induction of matrix metalloproteinase-9 (MMP-9) in vitro and in vivo, and thus enhances invasion and migration. We also show that fos plays a crucial role in the transcriptional activation of MMP-9 by S1P. In addition, activation of extracellular-signal-regulated kinases 1 and 2 (ERK1/2), p38 and alpha serine/threonine-protein kinase (Akt) are involved in the process of S1P-mediated induction of MMP-9 expression and invasion. Activation of the S1P receptor S1P(3) and G(alpha q) are required for S1P-induced invasive/migratory responses, suggesting that the enhancement of S1P-mediated invasiveness is triggered by the specific coupling of S1P(3) to the heterotrimeric G(alpha q) subunit. Activation of phospholipase C-beta(4) and intracellular Ca2+ release are required for S1P-induced MMP-9 upregulation. Taken together, this study demonstrated that S1P regulates MMP-9 induction and invasiveness through coupling of S1P(3) and G(alpha q) in MCF10A cells, thus providing a molecular basis for the crucial role of S1P in promoting breast cell invasion. | en_US |
dc.language.iso | en | en_US |
dc.publisher | COMPANY OF BIOLOGISTS LTD | en_US |
dc.subject | S1P | en_US |
dc.subject | Breast cell invasion | en_US |
dc.subject | MMP-9 | en_US |
dc.title | Sphingosine 1-phosphate regulates matrix metalloproteinase-9 expression and breast cell invasion through S1P3-Gaq coupling | en_US |
dc.type | Article | en_US |
dc.relation.no | 13 | - |
dc.relation.volume | 124 | - |
dc.identifier.doi | 10.1242/jcs.076794 | - |
dc.relation.page | 2220-2230 | - |
dc.relation.journal | JOURNAL OF CELL SCIENCE | - |
dc.contributor.googleauthor | Kim, Eun-Sook | - |
dc.contributor.googleauthor | Kim, Jong-Sook | - |
dc.contributor.googleauthor | Kim, Sang Geon | - |
dc.contributor.googleauthor | Hwang, Sejin | - |
dc.contributor.googleauthor | Lee, Chang Ho | - |
dc.contributor.googleauthor | Moon, Aree | - |
dc.relation.code | 2011204794 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF MEDICINE[S] | - |
dc.sector.department | DEPARTMENT OF MEDICINE | - |
dc.identifier.pid | jennysue | - |
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