Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 정일엽 | - |
dc.date.accessioned | 2018-02-12T04:50:09Z | - |
dc.date.available | 2018-02-12T04:50:09Z | - |
dc.date.issued | 2011-07 | - |
dc.identifier.citation | Biochemical and biophysical research communications,Vol.410 No.3 [2011],637-642 | en_US |
dc.identifier.issn | 0006-291X | - |
dc.identifier.uri | http://www.sciencedirect.com/science/article/pii/S0006291X11010023?_rdoc=1&_fmt=high&_origin=gateway&_docanchor=&md5=b8429449ccfc9c30159a5f9aeaa92ffb | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11754/36627 | - |
dc.description.abstract | NANOG is a homeodomain-containing transcription factor that is essential for the maintenance of pluripotency and self-renewal in embryonic stem cells. However, the molecular mechanisms underlying the regulation of NANOG expression in human cells remain largely unknown. Here, we investigated the role of Tcf/Lef response elements located in the enhancer of the human NANOG gene. We found that forced expression of Lef1 or beta-catenin stimulated human NANOG promoter activity, while shRNA-mediated knockdown of beta-catenin reduced Lef1-induced NANOG promoter activation. Deletion or mutation of the Tcf/Lef element within the enhancer region of the human NANOG gene completely abrogated Lef1-induced NANOG promoter activity. The results of a chromatin immunoprecipitation assay demonstrated that Lefl and beta-catenin bind to the Tcf/Lef element in the enhancer region of the NANOG gene. Forced expression of GSK-31 inhibited basal, Lefl -induced, and beta-catenin-induced NANOG promoter activity, while treatment with the GSK-3 beta inhibitor SB216763 resulted in the accumulation of beta-catenin and NANOG protein. Furthermore, DvI-1-induced NANOG promoter activity was abrogated by the expression of beta-catenin shRNA. Stable overexpression of Dvl-1 caused beta-catenin and NANOG to accumulate. These results indicate that the Tcf/Lef response element in the enhancer region of the human NANOG gene is able to stimulate NANOG gene transcription. (C) 2011 Elsevier Inc. All rights reserved. | en_US |
dc.description.sponsorship | This work was supported by the Basic Science Research Program (Grant No. 2009-0076856) and the Disease Network Research Program (Grant No. 20090084181) of the National Research Foundation of Korea (NRF), funded by the Ministry of Education. Science and Technology, Republic of Korea. | en_US |
dc.language.iso | en | en_US |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE, 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA | en_US |
dc.subject | NANOG | en_US |
dc.subject | Dvl-1 | en_US |
dc.subject | Tcf/Lef | en_US |
dc.subject | beta-Catenin | en_US |
dc.subject | GSK-3 beta | en_US |
dc.title | A Tcf/Lef element within the enhancer region of the human NANOG gene plays a role in promoter activation | en_US |
dc.type | Article | en_US |
dc.relation.no | 3 | - |
dc.relation.volume | 410 | - |
dc.identifier.doi | 10.1016/j.bbrc.2011.06.044 | - |
dc.relation.page | 637-642 | - |
dc.relation.journal | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | - |
dc.contributor.googleauthor | Kim, Chang Gun | - |
dc.contributor.googleauthor | Chung, Il-Yup | - |
dc.contributor.googleauthor | Lim, Yoongho | - |
dc.contributor.googleauthor | Lee, Young Han | - |
dc.contributor.googleauthor | Shin, Soon Young | - |
dc.relation.code | 2011201235 | - |
dc.sector.campus | S | - |
dc.sector.daehak | GRADUATE SCHOOL[S] | - |
dc.sector.department | DEPARTMENT OF BIONANOTECHNOLOGY | - |
dc.identifier.pid | iychu | - |
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