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Global Transcriptome Profiling of Genes that Are Differentially Regulated During Differentiation of Mouse Embryonic Neural Stem Cells into Astrocytes

Title
Global Transcriptome Profiling of Genes that Are Differentially Regulated During Differentiation of Mouse Embryonic Neural Stem Cells into Astrocytes
Author
이영식
Keywords
Neural stem cells; Astrocytes; Cell differentiation; TGF beta; WNTs; IN-VIVO; RNA-SEQ; EXPRESSION; SPECIFICATION; IDENTIFICATION; OLIGODENDROCYTES; PLURIPOTENT; INSIGHTS; CULTURES; DATABASE
Issue Date
2015-01
Publisher
HUMANA PRESS INC
Citation
JOURNAL OF MOLECULAR NEUROSCIENCE, v. 55, No. 1, Page. 109-125
Abstract
"Many genes are associated with the differentiation of neural stem cells (NSCs) into astrocytes, the most abundant and functionally diverse population of glial cells in the central nervous system, particularly in the brain. In the present study, we differentiated NSCs from the forebrain of embryonic day 14.5 mouse embryos into astrocytes over 1 and 7 days. We identified transcriptomes of NSCs and astrocytes using RNA sequencing and analyzed enriched gene networks, signal pathways, and ontology. To identify important regulators of differentiation, we performed gene clustering according to expression patterns and promoter CG types. Our data show that genes related to system development, including Fbln2, Bcan, Ncam1, Itih3, Tnr, and Vcan, regulate NSC differentiation through WNT/beta-catenin and epithelial to mesenchymal transition pathways. We identified many CG-rich promoter genes related to basic cellular maintenance such as transcription, translation, and structural components and CG-poor promoter genes that are highly associated with cell-type-specific functions or play important roles during development. Our study provides a foundation for further research on NSC differentiation and the future application of stem cells."
URI
https://link.springer.com/article/10.1007/s12031-014-0382-8http://hdl.handle.net/20.500.11754/35433
ISSN
0895-8696; 1559-1166
DOI
10.1007/s12031-014-0382-8
Appears in Collections:
COLLEGE OF SCIENCE AND CONVERGENCE TECHNOLOGY[E](과학기술융합대학) > MOLECULAR AND LIFE SCIENCE(분자생명과학과) > Articles
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