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Tcf7l1-mediated transcriptional regulation of Kruppel-like factor 4 gene

Title
Tcf7l1-mediated transcriptional regulation of Kruppel-like factor 4 gene
Author
이영식
Keywords
TCF-RE; Tcf7l1; beta-catenin; KLF4; transcription; CELL SELF-RENEWAL; EMBRYONIC STEM-CELLS; BETA-CATENIN BINDING; COLORECTAL-CANCER; DEFINED FACTORS; DNA-DAMAGE; TCF3; DIFFERENTIATION; PLURIPOTENCY; EXPRESSION
Issue Date
2015-01
Publisher
TAYLOR & FRANCIS LTD
Citation
ANIMAL CELLS AND SYSTEMS, v. 19, No. 1, Page. 16-29
Abstract
The transcription factor Kruppel-like factor 4 (KLF4) is highly expressed and plays an important role in maintaining stemness in embryonic stem cells (ES cells). However, how the expression of KLF4 is regulated has not been well documented. To elucidate the molecular mechanisms controlling the expression of human KLF4 (hKLF4) in ES cells, a 3685-bp DNA fragment upstream of the hKLF4 gene was isolated and the 5 '-regulatory region was characterized, from which two regions highly conserved with rodents were identified. In addition, one consensus binding site for members of the TCF family #TBS# was identified within each conserved region. Tcf7l1, a member of the TCF family, is highly expressed in ES cells and has been identified as a crucial regulator of the pluripotency gene regulatory network in ES cells. Therefore, the possible transcriptional mechanisms by which Tcf7l1 regulates hKLF4 expression in ES cells were investigated. In the present study, gel retardation showed that Tcf7l1 preferentially bound to the distal TBS element. In addition, overexpression of Tcf7l1 significantly increased the expression of endogenous KLF4 mRNA in both HEK293 #low nuclear beta-catenin# and HCT116 cells #high nuclear beta-catenin#. In both cell lines, the relative expression of KLF4 mRNA was correlated with the increased Tcf7l1 gene expression, but not with active beta-catenin activities. In transient transfection assays, the hKLF4-3685 construct showed 15-fold activity compared to a deletion construct #hKLF4-1033# in HCT116 cells, while significant activity was not observed in HEK293 cells. As the full promoter construct was active only in HCT116 cells, not HEK293 cells, endogenous Tcf4 may activate promoter with active beta-catenin. However, Tcf7l1 overexpression showed similar promoter activation in both cells. While Tcf7l1 stimulated the hKLF-3685 promoter by 4-6 fold, it did not stimulate the hKLF4-1033 construct at all in either of the cell lines, revealing that the Tcf7l1 responsive element must be present -3685 to -1033 upstream of the translation initiation site, which was consistent with the gel retardation data. Collectively, the present results demonstrated that distal TBS was essential for TCF-mediated transcriptional activation of the hKLF4 promoter. In addition, while TBS responds to Tcf4 in a Wnt/A beta -catenin-dependent manner, it responded to Tcf7l1 independent of active beta-catenin activity.
URI
http://www.tandfonline.com/doi/abs/10.1080/19768354.2014.991351http://hdl.handle.net/20.500.11754/35418
ISSN
1976-8354; 2151-2485
DOI
10.1080/19768354.2014.991351
Appears in Collections:
COLLEGE OF SCIENCE AND CONVERGENCE TECHNOLOGY[E](과학기술융합대학) > MOLECULAR AND LIFE SCIENCE(분자생명과학과) > Articles
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