Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 채필석 | - |
dc.date.accessioned | 2018-02-03T05:55:05Z | - |
dc.date.available | 2018-02-03T05:55:05Z | - |
dc.date.issued | 2011-03 | - |
dc.identifier.citation | JOURNAL OF PROTEOME RESEARCH, v. 10, NO 6, Page. 2703-2714 | en_US |
dc.identifier.issn | 1535-3893 | - |
dc.identifier.uri | https://pubs.acs.org/doi/abs/10.1021/pr100838x | - |
dc.description.abstract | The chromatophore membrane of the photosynthetic diazotroph Rhodospirillum rubrum is of vital importance for a number of central processes, including nitrogen fixation. Using a novel amphiphile, we have identified protein complexes present under different nitrogen availability conditions by the use of two-dimensional Blue Native/SDS-PAGE and NSI-LC-LTQ-Orbitrap mass spectrometry. We have identified several membrane protein complexes, including components of the ATP synthase, reaction center, light harvesting, and NADH dehydrogenase complexes. Additionally, we have identified differentially expressed proteins, such as subunits of the succinate dehydrogenase complex and other TCA cycle enzymes that are usually found in the cytosol, thus hinting at a possible association to the membrane in response to nitrogen deficiency. We propose a redox sensing mechanism that can influence the membrane subproteome in response to nitrogen availability. | en_US |
dc.description.sponsorship | This work was supported by grants from the Carl Tryggers Foundation to AN., from the Swedish Research Council to S.N., from Fundacao para a Ciencia e a Tecnologia (Portugal), through the PhD fellowship SERH/BD/23183/2005 to T.T.S., from the NIH grant P01 GM75913 to P.S.C. and S.H.G. and from the Lundbeck Foundation to S.R. We thank Dr. Jan-Willem Schmidt for excellent technical assistance with the sucrose density gradients and Western blots. | en_US |
dc.language.iso | en | en_US |
dc.publisher | AMER CHEMICAL SOC | en_US |
dc.subject | Rhodospirillum rubrum | en_US |
dc.subject | nitrogen metabolism | en_US |
dc.subject | Blue Native | en_US |
dc.subject | chromatophore subproteome | en_US |
dc.subject | amphiphile | en_US |
dc.subject | Orbitrap | en_US |
dc.title | Identification of Chromatophore Membrane Protein Complexes Formed under Different Nitrogen Availability Conditions in Rhodospirillum rubrum | en_US |
dc.type | Article | en_US |
dc.relation.no | 6 | - |
dc.relation.volume | 10 | - |
dc.identifier.doi | 10.1021/pr100838x | - |
dc.relation.page | 2703-2714 | - |
dc.relation.journal | JOURNAL OF PROTEOME RESEARCH | - |
dc.contributor.googleauthor | Selao, T.T. | - |
dc.contributor.googleauthor | Branca, R. | - |
dc.contributor.googleauthor | Chae, P.S. | - |
dc.contributor.googleauthor | Lehtio, J. | - |
dc.contributor.googleauthor | Gellman, S.H. | - |
dc.contributor.googleauthor | Rasmussen, S.G.F | - |
dc.contributor.googleauthor | Nordlund, S. | - |
dc.contributor.googleauthor | Noren, A. | - |
dc.relation.code | 2011212739 | - |
dc.sector.campus | S | - |
dc.sector.daehak | GRADUATE SCHOOL[S] | - |
dc.sector.department | DEPARTMENT OF BIONANOTECHNOLOGY | - |
dc.identifier.pid | pchae | - |
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