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Identification of RNA-Binding Proteins by RNA Ligand-based cDNA Expression Library Screening

Title
Identification of RNA-Binding Proteins by RNA Ligand-based cDNA Expression Library Screening
Author
김철근
Keywords
RNA-protein interaction; Phage display; Requiem (REQ/DPF2); CDNA library screening
Issue Date
2016-02
Publisher
Bio-protocol LLC
Citation
bio-protocol, v. 6, NO 2, Article number 1715, Page. 1-8
Abstract
We previously reported when a portion of the Requiem (REQ/DPF2) messenger ribonucleic acid (mRNA) 3’ untranslated region (3’UTR), referred to as G8, was overexpressed in K562 cells, β-globin expression was induced, suggesting that the 3’UTR of REQ mRNA plays a physiological role (Kim et al., 2014). To identify trans-acting factors that bind to the REQ 3’UTR, we describe the RNA ligand based cDNA expression library screening method. This protocol could be adapted to detect specific RNA-protein interactions. Following this method, we identified six positive clones in the initial round of screening and four pure clones after sib-screening. This protocol was originally published in Kim et al. (2014).
URI
http://www.bio-protocol.org/e1715http://hdl.handle.net/20.500.11754/33996
ISSN
2331-8325
DOI
10.21769/BioProtoc.1715
Appears in Collections:
COLLEGE OF NATURAL SCIENCES[S](자연과학대학) > LIFE SCIENCE(생명과학과) > Articles
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