방사선 조사된 다공성 PCL/PLLA 마이크로스피어의 생체적합성 및 특성 평가

Abstract

Porous microspheres have been used in various fields, which are tissue engineering and drug delivery etc. Porosity and size of microsphere have been developed by emulsion polymerization such as solvent evaporation, phase separation, spray drying and ultrasonic atomizer. In this study, microspheres were prepared by solvent evaporation using PCL and PLLA polymers, which are excellent biocompatibility and biodegradability. After irradiating the prepared microspheres, the two polymers were evaluated according to the effect of gamma-irradiation (50, 75 and 100 kGy, 10kGy/hr). Alendronate, which is a bisphosphonate drug, was used as a drug model, and it has been treat musculoskeletal disorders. Musculoskeletal disorders, including osteoporosis and osteoarthritis, are caused by inflammation and damage to muscles, blood vessels and nerves. They are occurred by extending life expectancy, material handling, workstation and repetitive motion injuries. In this paper, porous PCL/PLLA microspheres were prepared and irradiated with gamma ray. To confirm the morphology of the microspheres and the pores of the surface, we confirmed the porous structure by SEM images. The porous PLLA microspheres also confirmed the presence of pores in the interior structure of the microspheres in 2D and 3D images through XRM. It was confirmed that the porosity of the microspheres and the crystallinity of the PLLA microspheres were increased with increasing gamma irradiation dose. The microspheres containing alendronate showed stable release through drug release behavior and the cytotoxicity of the microspheres was confirmed to be non-toxic through cck-8 assay. Therefore, the irradiated porous PCL/PLLA microspheres can have a potential to be delivered drugs. Also, further studies are needed to establish manufacturing conditions with various sizes of the microspheres and pores by controlling diverse factors such as the speed of homogenizer, the content of the gelatin and the evaporation time of the solvent in the manufacturing process.