206 0

Radiotherapy diagnostic biomarkers in radioresistant human H460 lung cancer stem-like cells

Title
Radiotherapy diagnostic biomarkers in radioresistant human H460 lung cancer stem-like cells
Author
이수재
Keywords
Biomarker; cancer stem-like cells; diagnose; H460 lung cancer cells; proteomics; radioresistance; radiotherapy
Issue Date
2016-02
Publisher
TAYLOR & FRANCIS INC
Citation
CANCER BIOLOGY & THERAPY, v. 17, NO 2, Page. 208-218
Abstract
Tumor cell radioresistance is a major contributor to radiotherapy failure, highlighting the importance of identifying predictive biomarkers for radioresistance. In this work, we established a radioresistant H460 (RR-H460) cell line from parental radiosensitive H460 lung cancer cells by exposure to fractionated radiation. The radiation-resistant, anti-apoptotic phenotype of RR-H460 cell lines was confirmed by their enhanced clonogenic survival and increased expression of the radioresistance genes Hsp90 and Her-3. RR-H460 cells displayed characteristics of cancer stem-like cells (CSCs), including induction of the surface marker CD44 and stem cell markers Nanog, Oct4, and Sox2. RR-H460 cells also exhibited sphere formation and malignant behavior, further supporting a CSC phenotype. Using proteomic analyses, we identified 8 proteins that were up-regulated in RR-H460 CSC lines and therefore potentially involved in radioresistance and CSC-related biological processes. Notably, 4 of thesePAI-2, NOMO2, KLC4, and PLOD3have not been previously linked to radioresistance. Depletion of these individual genes sensitized RR-H460 cells to radiotoxicity and additively enhancing radiation-induced apoptosis. Our findings suggest the possibility of integrating molecular targeted therapy with radiotherapy as a strategy for resolving the radioresistance of lung tumors.
URI
http://www.tandfonline.com/doi/full/10.1080/15384047.2016.1139232http://hdl.handle.net/20.500.11754/31881
ISSN
1538-4047; 1555-8576
DOI
10.1080/15384047.2016.1139232
Appears in Collections:
COLLEGE OF NATURAL SCIENCES[S](자연과학대학) > LIFE SCIENCE(생명과학과) > Articles
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE