276 0

Full metadata record

DC FieldValueLanguage
dc.contributor.author주재범-
dc.date.accessioned2017-11-16T01:27:16Z-
dc.date.available2017-11-16T01:27:16Z-
dc.date.issued2016-01-
dc.identifier.citationBIOSENSORS & BIOELECTRONICS, v. 78, Page. 530-537en_US
dc.identifier.issn0956-5663-
dc.identifier.issn1873-4235-
dc.identifier.urihttp://www.sciencedirect.com/science/article/pii/S0956566315306503?via%3Dihub-
dc.identifier.urihttp://hdl.handle.net/20.500.11754/31445-
dc.description.abstractUser-friendly lateral flow (LF) strips have been extensively used for point-of-care (POC) self-diagnostics, but they have some limitations in their detection sensitivity and quantitative analysis because they only identify the high cut-off value of a biomarker by utilizing color changes that are detected with the naked eye. To resolve these problems associated with LF strips, we developed a novel surface-enhanced Raman scattering (SERS)-based LF assay for the quantitative analysis of a specific biomarker in the low concentration range. Herein, human immunodeficiency virus type 1 (HIV-1) DNA was chosen as the specific biomarker. Raman reporter-labeled gold nanoparticles (AuNPs) were employed as SERS nano tags for targeting and detecting the HIV-1 DNA marker, as opposed to using bare AuNPs in LF strips. It was possible to quantitatively analyze HIV-1 DNA with high sensitivity by monitoring the characteristic Raman peak intensity of the DNA-conjugated AuNPs. Under optimized conditions, the detection limit of our SERS-based lateral flow assay was 0.24 pg/mL, which was at least 1000 times more sensitive compared to colorimetric or fluorescent detection methods. These results demonstrate the potential feasibility of the proposed SERS-based lateral flow assay to quantitatively detect a broad range of genetic diseases with high sensitivity. (C) 2015 Elsevier B.V. All rights reserved.en_US
dc.description.sponsorshipThe National Research Foundation of Korea supported this work through Grant numbers 2008-0061891 and 2009-00426. The Nano Material Technology Development Program also supported this work, through the National Research Foundation of Korea, funded by the Ministry of Science, ICT and Future Planning (2012M3A7B4035288). This work was partially supported by a grant of the National Natural Science Foundation of China through Grant number 21275158.en_US
dc.language.isoenen_US
dc.publisherELSEVIER ADVANCED TECHNOLOGYen_US
dc.subjectHuman immunodeficiency virus type 1 (HIV-1)en_US
dc.subjectSurface-enhanced Raman scatteringen_US
dc.subjectGold nanoparticlesen_US
dc.subjectDNAen_US
dc.subjectLateral flow assayen_US
dc.titleA SERS-based lateral flow assay biosensor for highly sensitive detection of HIV-1 DNAen_US
dc.typeArticleen_US
dc.relation.volume78-
dc.identifier.doi10.1016/j.bios.2015.11.099-
dc.relation.page530-537-
dc.relation.journalBIOSENSORS & BIOELECTRONICS-
dc.contributor.googleauthorFu, Xiuli-
dc.contributor.googleauthorCheng, Ziyi-
dc.contributor.googleauthorYu, Jimin-
dc.contributor.googleauthorChoo, Priscilla-
dc.contributor.googleauthorChen, Lingxin-
dc.contributor.googleauthorChoo, Jaebum-
dc.relation.code2016001453-
dc.sector.campusS-
dc.sector.daehakGRADUATE SCHOOL[S]-
dc.sector.departmentDEPARTMENT OF BIONANOTECHNOLOGY-
dc.identifier.pidjbchoo-
Appears in Collections:
GRADUATE SCHOOL[S](대학원) > BIONANOTECHNOLOGY(바이오나노학과) > Articles
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE