Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 박장환 | - |
dc.date.accessioned | 2017-11-09T01:26:21Z | - |
dc.date.available | 2017-11-09T01:26:21Z | - |
dc.date.issued | 2016-01 | - |
dc.identifier.citation | JOURNAL OF BIOLOGICAL CHEMISTRY, v. 291, NO 2, Page. 752-761 | en_US |
dc.identifier.issn | 0021-9258 | - |
dc.identifier.issn | 1083-351X | - |
dc.identifier.uri | http://www.jbc.org/content/291/2/752 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11754/30589 | - |
dc.description.abstract | We have previously reported that Ahnak-mediated TGF beta signaling leads to down-regulation of c-Myc expression. Here, we show that inhibition of Ahnak can promote generation of induced pluripotent stem cells (iPSC) via up-regulation of endogenous c-Myc. Consistent with the c-Myc inhibitory role of Ahnak, mouse embryonic fibroblasts from Ahnak-deficient mouse (Ahnak(-/-) MEF) show an increased level of c-Myc expression compared with wild type MEF. Generation of iPSC with just three of the four Yamanaka factors, Oct4, Sox2, and Klf4 (hereafter 3F), was significantly enhanced in Ahnak(-/-) MEF. Similar results were obtained when Ahnak-specific shRNA was applied to wild type MEF. Of note, expression of Ahnak was significantly induced during the formation of embryoid bodies from embryonic stem cells, suggesting that Ahnak-mediated c-Myc inhibition is involved in embryoid body formation and the initial differentiation of pluripotent stem cells. The iPSC from 3F-infected Ahnak(-/-) MEF cells (Ahnak(-/-) - iPSC-3F) showed expression of all stem cell markers examined and the capability to form three primary germ layers. Moreover, injection of Ahnak(-/-) - iPSC-3F into athymic nude mice led to development of teratoma containing tissues from all three primary germ layers, indicating that iPSC from Ahnak(-/-) MEF are bona fide pluripotent stem cells. Taken together, these data provide evidence for a new role for Ahnak in cell fate determination during development and suggest that manipulation of Ahnak and the associated signaling pathway may provide a means to regulate iPSC generation. | en_US |
dc.description.sponsorship | This work was supported by National Research Foundation of Korea Grant 2012R1A5A1048236, by Bio & Medical Technology Development Program Grant 2012M3A9B4028785, Redoxomics Grant 2012M3A9C5048708 funded by the Ministry of Science, ICT & Future Planning. This work was also supported by Grant A120262 from the Ministry of Health & Welfare, Republic of Korea. The authors declare that they have no conflicts of interest with the contents of this article. | en_US |
dc.language.iso | en | en_US |
dc.publisher | AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC | en_US |
dc.subject | KRUPPEL-LIKE FACTORS | en_US |
dc.subject | HUMAN SOMATIC-CELLS | en_US |
dc.subject | PROTEIN-KINASE-C | en_US |
dc.subject | TRANSCRIPTION FACTOR | en_US |
dc.subject | IPSC GENERATION | en_US |
dc.subject | FIBROBLASTS | en_US |
dc.subject | MOUSE | en_US |
dc.subject | INHIBITION | en_US |
dc.subject | ACTIVATION | en_US |
dc.subject | EFFICIENCY | en_US |
dc.title | Regulation of c-Myc Expression by Ahnak Promotes Induced Pluripotent Stem Cell Generation | en_US |
dc.type | Article | en_US |
dc.relation.no | 2 | - |
dc.relation.volume | 291 | - |
dc.identifier.doi | 10.1074/jbc.M115.659276 | - |
dc.relation.page | 752-761 | - |
dc.relation.journal | JOURNAL OF BIOLOGICAL CHEMISTRY | - |
dc.contributor.googleauthor | Lim, Hee Jung | - |
dc.contributor.googleauthor | Kim, Jusong | - |
dc.contributor.googleauthor | Park, Chang-Hwan | - |
dc.contributor.googleauthor | Lee, Sang A. | - |
dc.contributor.googleauthor | Lee, Man Ryul | - |
dc.contributor.googleauthor | Kim, Kye-Seong | - |
dc.contributor.googleauthor | Kim, Jaesang | - |
dc.contributor.googleauthor | Bae, Yun Soo | - |
dc.relation.code | 2016002045 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF MEDICINE[S] | - |
dc.sector.department | DEPARTMENT OF MEDICINE | - |
dc.identifier.pid | chshpark | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.