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Peptide micelle-mediated curcumin delivery for protection of islet beta-cells under hypoxia

Title
Peptide micelle-mediated curcumin delivery for protection of islet beta-cells under hypoxia
Author
이민형
Keywords
Apoptosis; curcumin; hypoxia; peptide micelle; pancreatic beta-cell
Issue Date
2016-01
Publisher
TAYLOR & FRANCIS LTD
Citation
JOURNAL OF DRUG TARGETING, v. 24, NO 7, Page. 618-623
Abstract
Islet transplantation is one of many therapeutic approaches for the treatment of diabetes. During transplant procedures, the isolated islets are subjected to hypoxic conditions, and undergo the apoptotic process. Curcumin has a cytoprotective effect, and may therefore be useful for the protection of islets under hypoxia. However, curcumin is hydrophobic, and an efficient curcumin carrier is required for effective treatment. In this study, R3V6 peptide micelles, composed of a 3-arginine stretch and 6-valine stretch, were evaluated as a curcumin carrier to INS-1 insulinoma cells. Curcumin was loaded into R3V6 micelles at a weight ratio of 10: 3 (R3V6: curcumin). The size and surface charge of the curcumin-loaded R3V6 micelles (R3V6-curcumin) were approximately 250nm and 17.49 mV, respectively. R3V6-curcumin delivered curcumin to the INS-1 cells more efficiently than either curcumin alone or a simple mixture of R3V6 and curcumin. MTT assay indicated that under hypoxia, R3V6-curcumin protected INS-1 cells more efficiently than curcumin alone. TUNEL and reactive oxygen species (ROS) assays suggested that R3V6-curcumin reduced INS-1 cell apoptosis under hypoxia. These results demonstrate that R3V6 peptide micelles are an effective carrier of curcumin, and that R3V6-curcumin may improve the viability of pancreatic beta-cells in islet transplantation.
URI
http://www.tandfonline.com/doi/full/10.3109/1061186X.2015.1132220http://hdl.handle.net/20.500.11754/30526
ISSN
1061-186X; 1029-2330
DOI
10.3109/1061186X.2015.1132220
Appears in Collections:
COLLEGE OF ENGINEERING[S](공과대학) > BIOENGINEERING(생명공학과) > Articles
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