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dc.contributor.author이수재-
dc.date.accessioned2017-10-24T04:57:00Z-
dc.date.available2017-10-24T04:57:00Z-
dc.date.issued2015-12-
dc.identifier.citationEUROPEAN JOURNAL OF CELL BIOLOGY, v. 94, NO 12, Page. 653-660en_US
dc.identifier.issn0171-9335-
dc.identifier.issn1618-1298-
dc.identifier.urihttp://www.sciencedirect.com/science/article/pii/S017193351530008X?via%3Dihub-
dc.identifier.urihttp://hdl.handle.net/20.500.11754/30222-
dc.description.abstractDespite many studies of the effect of ionizing radiation, biological mechanisms of action might differ greatly depend on dose, dose rate, and cell type. This study was performed to explore the effects of low- and high-dose radiation in human immune cell lines. We examined cell sensitivity after irradiation with 0.05, 0.1, or 2 Gy in two normal cell lines and three tumor cell lines. Low-dose radiation of 0.05 and 0.1 Gy had no effect on cell survival in any tested cell line, with the exception of IM-9 cells, whose viability was transiently increased. However, IM-9 and C1R-sB7 cells were very sensitive to high-dose radiation-induced cell death, whereas Jurkat and JM1 cells showed moderate sensitivity, and THP-1 cells were completely resistant. This radiosensitivity was correlated with basal ART activation, which is induced by phosphorylation. In radiosensitive IM-9 cells, priming with chronic low-dose irradiation blocked cell death induced by high-dose radiation challenge via inhibition of caspase activation and PARP cleavage. ART phosphorylation was not altered in IM-9 cells, but ERK phosphorylation was greatly elevated immediately after chronic low-dose irradiation. Taken together, our results suggest that the different responses of normal and tumor cells to low-dose and high-dose radiation depend on ART activation, which is regulated by protein phosphatase 2 (PP2A). In radiosensitive normal cells lacking basal ART activity, chronic low-dose radiation increases activation of the ERK pathway, which plays an important role in the adaptive response to radiation, providing a very important insight into understanding the effects of ionizing radiation on health. (C) 2015 Elsevier GmbH. All rights reserved.en_US
dc.description.sponsorshipThis work was supported by grant no. 20131610101840 from the Ministry of Trade, Industry & Energy (MOTIE), Republic of Korea.en_US
dc.language.isoenen_US
dc.publisherELSEVIER GMBHen_US
dc.subjectIonizing radiationen_US
dc.subjectLow-doseen_US
dc.subjectAKTen_US
dc.subjectERKen_US
dc.subjectApoptosisen_US
dc.titleRole of AKT and ERK pathways in controlling sensitivity to ionizing radiation and adaptive response induced by low-dose radiation in human immune cellsen_US
dc.typeArticleen_US
dc.relation.no12-
dc.relation.volume94-
dc.identifier.doi10.1016/j.ejcb.2015.08.003-
dc.relation.page653-660-
dc.relation.journalEUROPEAN JOURNAL OF CELL BIOLOGY-
dc.contributor.googleauthorPark, Hyung Sun-
dc.contributor.googleauthorYou, Ga Eun-
dc.contributor.googleauthorYang, Kwang Hee-
dc.contributor.googleauthorKim, Ji Young-
dc.contributor.googleauthorAn, Sungkwan-
dc.contributor.googleauthorSong, Jie-Young-
dc.contributor.googleauthorLee, Su-Jae-
dc.contributor.googleauthorLim, Young-Khi-
dc.contributor.googleauthorNam, Seon Young-
dc.relation.code2015000866-
dc.sector.campusS-
dc.sector.daehakCOLLEGE OF NATURAL SCIENCES[S]-
dc.sector.departmentDEPARTMENT OF LIFE SCIENCE-
dc.identifier.pidsj0420-
Appears in Collections:
COLLEGE OF NATURAL SCIENCES[S](자연과학대학) > LIFE SCIENCE(생명과학과) > Articles
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