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Synergic hypocholesterolaemic effect of n-3 PUFA and oestrogen by modulation of hepatic cholesterol metabolism in female rats

Title
Synergic hypocholesterolaemic effect of n-3 PUFA and oestrogen by modulation of hepatic cholesterol metabolism in female rats
Author
박용순
Keywords
Cholesterol metabolism; Oestrogen; n-3 PUFA; Ovariectomised rats; Proprotein convertase subtilisin/kexin type 9
Issue Date
2015-12
Publisher
CAMBRIDGE UNIV PRESS
Citation
BRITISH JOURNAL OF NUTRITION, v. 114, NO 11, Page. 1766-1773
Abstract
n-3 PUFA such as EPA and DHA as well as oestrogen have been reported to decrease blood levels of cholesterol, but their underlying mechanism is unclear. The purpose of this study was to determine the effects of the combination of n-3 PUFA supplementation and oestrogen injection on hepatic cholesterol metabolism. Rats were fed a modified AIN-93G diet with 0, 1 or 2 % n-3 PUFA (EPA+DHA) relative to the total energy intake for 12 weeks. Rats were surgically ovariectomised at week 8, and, after 1-week recovery, rats were injected with 17 beta-oestradiol-3-benzoate (E-2) or maize oil for the last 3 weeks. Supplementation with n-3 PUFA and E-2 injection significantly increased the ratio of the hepatic expression of phosphorylated AMP activated protein kinase (p-AMPK):AMP activated protein kinase (AMPK) and decreased sterol regulatory element-binding protein-2, 3-hydroxy-3-methylglutaryl coenzyme A reductase and proprotein convertase subtilisin/kexin type 9. Supplementation with n-3 PUFA increased hepatic expression of cholesterol 7 alpha-hydroxylase (CYP7A1), sterol 12 alpha-hydroxylase (CYP8B1) and sterol 27-hydroxylase (CYP27A1); however, E-2 injection decreased CYP7A1 and CYP8B1 but not CYP27A1. Additionally, E-2 injection increased hepatic expression of oestrogen receptor-alpha and beta. In conclusion, n-3 PUFA supplementation and E-2 injection had synergic hypocholesterolaemic effects by down-regulating hepatic cholesterol synthesis (n-3 PUFA and oestrogen) and up-regulating bile acid synthesis (n-3 PUFA) in ovariectomised rats.
URI
https://www.cambridge.org/core/journals/british-journal-of-nutrition/article/synergic-hypocholesterolaemic-effect-of-n3-pufa-and-oestrogen-by-modulation-of-hepatic-cholesterol-metabolism-in-female-rats/0282C994BBF9382F0F49992D9009F196http://hdl.handle.net/20.500.11754/30216
ISSN
0007-1145; 1475-2662
DOI
10.1017/S0007114515003517
Appears in Collections:
COLLEGE OF HUMAN ECOLOGY[S](생활과학대학) > FOOD & NUTRITION(식품영양학과) > Articles
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