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dc.contributor.authorHeese, Klaus-
dc.date.accessioned2017-10-17T02:35:34Z-
dc.date.available2017-10-17T02:35:34Z-
dc.date.issued2015-12-
dc.identifier.citationJOURNAL OF FOOD BIOCHEMISTRY, v. 39, NO 6, Page. 754-764en_US
dc.identifier.issn0145-8884-
dc.identifier.issn1745-4514-
dc.identifier.urihttp://onlinelibrary.wiley.com/doi/10.1111/jfbc.12188/abstract-
dc.identifier.urihttp://hdl.handle.net/20.500.11754/30059-
dc.description.abstractManganese peroxidase (MnP) is necessary in fruit juice clarification for the degradation of phenolic compounds. In the present study, MnP was produced using basal medium supplemented with tamarind shell (1% w/v), ethanol (2% v/v) and gallic acid (1 mM) to a maximum activity of 65 U/mL. A 43.0 kDa MnP was purified by ammonium sulfate fractionation, Sephadex G-100 and DEAE-cellulose column chromatography with 6.5-fold and 13% yield. The optimal activity of the purified MnP was achieved at pH 5.5 and temperature of 45C with 2,6dimethoxyphenol as substrate. The inhibitors sodium azide, cyanide and EDTA inhibited the MnP activity up to 100, 93 and 84%, respectively. MnP showed high activity and stability in the presence of different metal ions, surfactants and organic solvents. Clarification of different fruit juices with MnP showed its potential application in the food and beverage industry.en_US
dc.description.sponsorshipThe authors thank the University of Madras Grants Commission for providing the scholarship to T.M. through Science for Meritorious Students, and Dr. R. Rengasamy, CAS in Botany, University of Madras, for providing the adequate laboratory facility to carry out the research. This study was supported by Hanyang University.en_US
dc.language.isoenen_US
dc.publisherWILEY-BLACKWELLen_US
dc.subjectPHANEROCHAETE-CHRYSOSPORIUMen_US
dc.subjectLIGNINOLYTIC ENZYMESen_US
dc.subjectORGANIC-SOLVENTSen_US
dc.subjectPURIFICATIONen_US
dc.subjectLACCASEen_US
dc.subjectBIODEGRADATIONen_US
dc.subjectPROTEINSen_US
dc.subjectDECOLORIZATIONen_US
dc.subjectFERMENTATIONen_US
dc.subjectETHANOLen_US
dc.titleCHARACTERIZATION OF A SOLVENT-TOLERANT MANGANESE PEROXIDASE (MnP) FROM GANODERMA LUCIDUM AND ITS APPLICATION IN FRUIT JUICE CLARIFICATIONen_US
dc.typeArticleen_US
dc.relation.no6-
dc.relation.volume39-
dc.identifier.doi10.1111/jfbc.12188-
dc.relation.page754-764-
dc.relation.journalJOURNAL OF FOOD BIOCHEMISTRY-
dc.contributor.googleauthorManavalan, Tamilvendan-
dc.contributor.googleauthorManavalan, Vetriselvan-
dc.contributor.googleauthorThangavelu, Kalaichelvan P.-
dc.contributor.googleauthorKutzner, Arne-
dc.contributor.googleauthorHeese, Klaus-
dc.relation.code2015000405-
dc.sector.campusS-
dc.sector.daehakGraduate School of Biomedical Science & Engineering[S]-
dc.identifier.pidklaus-
dc.identifier.researcherIDH-3128-2013-
dc.identifier.orcidhttp://orcid.org/0000-0002-0027-6993-
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GRADUATE SCHOOL OF BIOMEDICAL SCIENCE AND ENGINEERING[S](의생명공학전문대학원) > ETC
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