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dc.contributor.author윤채옥-
dc.date.accessioned2017-10-17T02:26:32Z-
dc.date.available2017-10-17T02:26:32Z-
dc.date.issued2015-12-
dc.identifier.citationBIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v. 468, NO 1-2, Page. 92-98en_US
dc.identifier.issn0006-291X-
dc.identifier.issn1090-2104-
dc.identifier.urihttp://www.sciencedirect.com/science/article/pii/S0006291X15308561-
dc.identifier.urihttp://hdl.handle.net/20.500.11754/30057-
dc.description.abstractDevelopmental endothelial locus-1 (Del-1) is an endogenous anti-inflammatory molecule that is highly expressed in the lung and the brain and limits leukocyte migration to these tissues. We previously reported that the expression of Del-1 is positively regulated by p53 in lung endothelial cells. Although several reports have implicated the altered expression of Del-1 gene in cancer patients, little is known about its role in tumor cells. We here investigated the effect of Del-1 on the features of human lung carcinoma cells. Del-1 mRNA was found to be significantly decreased in the human lung adenocarcinoma cell lines A549 (containing wild type of p53), H1299 ( for p53) and EKVX (mutant p53), compared to in human normal lung epithelial BEAS-2B cells and MRC-5 fibroblasts. The decrease of Del-1 expression was dependent on the p53 activity in the cell lines, but not on the expression of p53. Neither treatment with recombinant human Del-1 protein nor the introduction of adenovirus expressing Del-1 altered the expression of the apoptosis regulators BAX, PUMA and Bcl-2. Unexpectedly, the adenovirus-mediated overexpression of Del-1 gene into the lung carcinoma cell lines promoted proliferation and invasion of the lung carcinoma cells, as revealed by BrdU incorporation and transwell invasion assays, respectively. In addition, overexpression of the Del-1 gene enhanced features of epithelial mesenchymal transition (EMT), such as increasing vimentin while decreasing E-cadherin in A549 cells, and increases in the level of Slug, an EMT-associated transcription regulator. Our findings demonstrated for the first time that there are deleterious effects of high levels of Del-1 in lung carcinoma cells, and suggest that Del-1 may be used as a diagnostic or prognostic marker for cancer progression, and as a novel therapeutic target for lung carcinoma. (C) 2015 Elsevier Inc. All rights reserved.en_US
dc.description.sponsorshipThis work was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology of Korea (2011-0014447). We thank Dr. Kyung-Chul Choi for providing the A549 cell line, Dr. Seong Who Kim for the H1299 cell line, Dr. You Sook Cho for the MRC-5 and BEAS-2B cell lines, and Dr. Seok-Yong Choi for critical reading of the manuscript and encouragement.en_US
dc.language.isoenen_US
dc.publisherACADEMIC PRESS INC ELSEVIER SCIENCEen_US
dc.subjectLung carcinoma cellsen_US
dc.subjectTumor cell invasionen_US
dc.subjectTumor cell proliferationen_US
dc.subjectDel-1 (Developmental Endothelial Locus-1)en_US
dc.titleDel-1 overexpression potentiates lung cancer cell proliferation and invasionen_US
dc.typeArticleen_US
dc.relation.no1-2-
dc.relation.volume468-
dc.identifier.doi10.1016/j.bbrc.2015.10.159-
dc.relation.page92-98-
dc.relation.journalBIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS-
dc.contributor.googleauthorLee, Seung-Hwan-
dc.contributor.googleauthorKim, Dong-Young-
dc.contributor.googleauthorJing, Feifeng-
dc.contributor.googleauthorKim, Hyesoon-
dc.contributor.googleauthorYun, Chae-Ok-
dc.contributor.googleauthorHan, Deok-Jong-
dc.contributor.googleauthorChoi, Eun Young-
dc.relation.code2015001367-
dc.sector.campusS-
dc.sector.daehakCOLLEGE OF ENGINEERING[S]-
dc.sector.departmentDEPARTMENT OF BIOENGINEERING-
dc.identifier.pidchaeok-
dc.identifier.orcidhttp://orcid.org/0000-0002-9466-4531-
Appears in Collections:
COLLEGE OF ENGINEERING[S](공과대학) > BIOENGINEERING(생명공학과) > Articles
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