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dc.contributor.authorRamakrishna, Suresh-
dc.date.accessioned2017-10-16T02:26:46Z-
dc.date.available2017-10-16T02:26:46Z-
dc.date.issued2015-12-
dc.identifier.citationBIOCHIMICA ET BIOPHYSICA ACTA-REVIEWS ON CANCER, v. 1856, NO 2, Page. 234-243en_US
dc.identifier.issn0304-419X-
dc.identifier.issn0006-3002-
dc.identifier.urihttp://www.sciencedirect.com/science/article/pii/S0304419X15000700?via%3Dihub-
dc.identifier.urihttp://hdl.handle.net/20.500.11754/30033-
dc.description.abstractWhile human gene therapy has gained significant attention for its therapeutic promise, CRISPR/Cas9 technology has made a breakthrough as an efficient genome editing tool by emulating prokaryotic immune defense mechanisms. Although many studies have found that CRISPR/Cas9 technology is more efficient, specific and manipulable than previous generations of gene editing tools, it can be further improved by elevating its overall efficiency in a higher frequency of genome modifications and reducing its off-target effects. Here, we review the development of CRISPR/Cas9 technology, focusing on enhancement of its sequence specificity, reduction of off-target effects and delivery systems. Moreover, we describe recent successful applications of CRISPR/Cas9 technology in laboratory and clinical studies. (C) 2015 Elsevier B.V. All rights reserved.en_US
dc.description.sponsorshipWe would like to thank all of Suds laboratory members for their helpful discussions. This study was supported by a grant from the Hanyang University (201500000000436), the Korean Health Technology R&D Project, Ministry of Health and Welfare, Republic of Korea (HI14C2019 (Medistar program)), and the National Research Foundation of Korea (2014R1A1A1A05006189, 2011-0019357, 2013M3A9B4076544, 2015R1D1A1A01060907 and 2015H1D3A1036065).en_US
dc.language.isoenen_US
dc.publisherELSEVIER SCIENCE BVen_US
dc.subjectDelivery methodsen_US
dc.subjectGenome editingen_US
dc.subjectReduced off-target effectsen_US
dc.subjectSequence specificityen_US
dc.subjectTherapeuticsen_US
dc.subjectCRISPR/Cas9 applicationsen_US
dc.titleCRISPR/Cas9 system as an innovative genetic engineering tool: Enhancements in sequence specificity and delivery methodsen_US
dc.typeArticleen_US
dc.relation.no2-
dc.relation.volume1856-
dc.identifier.doi10.1016/j.bbcan.2015.09.003-
dc.relation.page234-243-
dc.relation.journalBIOCHIMICA ET BIOPHYSICA ACTA-REVIEWS ON CANCER-
dc.contributor.googleauthorJo, Young-Il-
dc.contributor.googleauthorSuresh, Bharathi-
dc.contributor.googleauthorKim, Hyongbum-
dc.contributor.googleauthorRamakrishna, Suresh-
dc.relation.code2015002459-
dc.sector.campusS-
dc.sector.daehakGraduate School of Biomedical Science & Engineering[S]-
dc.identifier.pidsuri28-
dc.identifier.orcidhttp://orcid.org/0000-0002-4038-1085-
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GRADUATE SCHOOL OF BIOMEDICAL SCIENCE AND ENGINEERING[S](의생명공학전문대학원) > ETC
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