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Cas-Designer: a web-based tool for choice of CRISPR-Cas9 target sites

Title
Cas-Designer: a web-based tool for choice of CRISPR-Cas9 target sites
Author
배상수
Keywords
RNA-GUIDED ENDONUCLEASES; NUCLEASES; CRISPR/CAS9; SYSTEMS; CELLS; DNA
Issue Date
2015-12
Publisher
OXFORD UNIV PRESS
Citation
BIOINFORMATICS, v. 31, NO 24, Page. 4014-4016
Abstract
We present Cas-Designer, a user-friendly program to aid researchers in choosing appropriate target sites in a gene of interest for type II CRISPR/Cas-derived RNA-guided endonucleases, which are now widely used for biomedical research and biotechnology. Cas-Designer rapidly provides the list of all possible guide RNA sequences in a given input DNA sequence and their potential off-target sites including bulge-type sites in a genome of choice. In addition, the program assigns an out-of-frame score to each target site to help users choose appropriate sites for gene knockout. Cas-Designer shows the results in an interactive table and provides user-friendly filter functions.
URI
https://academic.oup.com/bioinformatics/article-lookup/doi/10.1093/bioinformatics/btv537http://hdl.handle.net/20.500.11754/29575
ISSN
1367-4803; 1460-2059
DOI
10.1093/bioinformatics/btv537
Appears in Collections:
COLLEGE OF NATURAL SCIENCES[S](자연과학대학) > CHEMISTRY(화학과) > Articles
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