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dc.contributor.authorKUTZNER, ARNE HOLGER-
dc.date.accessioned2017-09-13T07:33:03Z-
dc.date.available2017-09-13T07:33:03Z-
dc.date.issued2015-11-
dc.identifier.citationBIOTECHNOLOGY LETTERS, v. 37, NO 12, Page. 2403-2409en_US
dc.identifier.issn0141-5492-
dc.identifier.issn1573-6776-
dc.identifier.urihttps://link.springer.com/article/10.1007%2Fs10529-015-1937-7-
dc.identifier.urihttp://hdl.handle.net/20.500.11754/29116-
dc.description.abstractObjective Laccase is industrially important but a major challenge is the production of an ideal laccase with suitable physicochemical properties to tolerate temperature, surfactants, metal ions and solvents towards its potential application in bioremediation. Results A laccase with a molecular mass of 43 kDa was purified from Pleurotus sp. MAK-II. It was optimally active at pH 4.5 and 60 degrees C using ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) as substrate. The laccase was susceptible to NaN3 and NaCN. Activity was strongly enhanced by Cu2+, Mg2+ and Ca2+. The purified laccase showed stability towards various surfactants and solvents and decolorized, in the presence of violuric acid as redox mediator, the diazo dye Congo Red and the anthraquinone dye Remazol Brilliant Blue R to the extent of 96 and 72 %, respectively. Conclusion The ideal physicochemical properties of Pleurotus sp. MAK-II-derived laccase suggest that it could be effectively used in the textile dye industry.en_US
dc.description.sponsorshipThe authors thank the University of Madras Grants Commission for providing the scholarship to A.M. through CSIR-SRF and Dr. N. Anand, CAS in Botany, University of Madras, for providing the adequate laboratory facility to carry out the research. This study was supported by Hanyang University.en_US
dc.language.isoenen_US
dc.publisherSPRINGERen_US
dc.subjectCongo Reden_US
dc.subjectLaccaseen_US
dc.subjectPleurotus sp.en_US
dc.subjectRemazol Brilliant Blue Ren_US
dc.subjectVioluric aciden_US
dc.titleCharacterization of a solvent, surfactant and temperature-tolerant laccase from Pleurotus sp MAK-II and its dye decolorizing propertyen_US
dc.typeArticleen_US
dc.relation.no12-
dc.relation.volume37-
dc.identifier.doi10.1007/s10529-015-1937-7-
dc.relation.page2403-2409-
dc.relation.journalBIOTECHNOLOGY LETTERS-
dc.contributor.googleauthorManavalan, Arulmani-
dc.contributor.googleauthorManavalan, Tamilvendan-
dc.contributor.googleauthorMurugesan, Kumarasamy-
dc.contributor.googleauthorKutzner, Arne-
dc.contributor.googleauthorThangavelu, Kalaichelvan P.-
dc.contributor.googleauthorHeese, Klaus-
dc.relation.code2015003603-
dc.sector.campusS-
dc.sector.daehakCOLLEGE OF ENGINEERING[S]-
dc.sector.departmentDEPARTMENT OF INFORMATION SYSTEMS-
dc.identifier.pidkutzner-
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COLLEGE OF ENGINEERING[S](공과대학) > INFORMATION SYSTEMS(정보시스템학과) > Articles
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