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dc.contributor.author황승용-
dc.date.accessioned2017-09-12T01:07:11Z-
dc.date.available2017-09-12T01:07:11Z-
dc.date.issued2015-11-
dc.identifier.citationJOURNAL OF ELECTROANALYTICAL CHEMISTRY, v. 756, Page. 118-123en_US
dc.identifier.issn1572-6657-
dc.identifier.issn1873-2569-
dc.identifier.urihttp://www.sciencedirect.com/science/article/pii/S1572665715300734?via%3Dihub-
dc.identifier.urihttp://hdl.handle.net/20.500.11754/29068-
dc.description.abstractConventional cancer diagnostic techniques are not suitable for point-of-care testing because they are usually expensive, time consuming, labor intensive, and require large equipment. This paper presents a proof-of-concept biochip for point-of-care testing based on multiplex electrochemical detection. The microfluidic biochip is composed of inexpensive photoresist-polydimethylsiloxane layers over a glass substrate. The substrate has an Au working electrode and a Pt counter/reference electrode. A microchannel was fabricated in the photoresist layer and a micromixer was fabricateclin the polydimethylsiloxane layer. Matrix metalloproteinases (MMPs) 2 and MMP7 are used as biomarkers for ovarian and colorectal cancer diagnosis. An unlabeled Peptide specific to MMPs is immobilized on an Au electrode and then impedance was measured using electrochemical impedance spectroscopy with electrolytes. As the MMP sample is injected into the biochip, the peptide is cleaved by enzyme hydrolysis and the impedance changes. MMP2 and MMP7 are quantitatively detected concurrently by measuring the variations of impedance. Using model samples, the detection ranges of the biochip were 0.1-400 ng/mL and 0.001-100 ng/mL for MMP2 and MMP7, respectively, and the overall assay time was reduced to about 1 h. The results indicate that a similar MMP-based electrochemical multiplex biochip could be used for the point-of-care diagnosis of many forms of cancer. (C) 2015 Elsevier B.V. All rights reserved.en_US
dc.description.sponsorshipThis research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2010-0010706).en_US
dc.language.isoenen_US
dc.publisherELSEVIER SCIENCE SAen_US
dc.subjectCancer diagnosisen_US
dc.subjectElectrochemical impedance spectroscopyen_US
dc.subjectEnzyme hydrolyzationen_US
dc.subjectLabel-free peptideen_US
dc.subjectPDMS-glass chipen_US
dc.titleMicrofluidic multiplex biochip based on a point-of-care electrochemical detection system for matrix metalloproteinasesen_US
dc.typeArticleen_US
dc.relation.volume756-
dc.identifier.doi10.1016/j.jelechem.2015.08.015-
dc.relation.page118-123-
dc.relation.journalJOURNAL OF ELECTROANALYTICAL CHEMISTRY-
dc.contributor.googleauthorHwang, Seung Yong-
dc.contributor.googleauthorSeo, In Jae-
dc.contributor.googleauthorLee, Seung Yong-
dc.contributor.googleauthorAhn, Yoomin-
dc.relation.code2015001788-
dc.sector.campusS-
dc.sector.daehakGRADUATE SCHOOL[S]-
dc.sector.departmentDEPARTMENT OF BIONANOTECHNOLOGY-
dc.identifier.pidsyhwang-
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GRADUATE SCHOOL[S](대학원) > BIONANOTECHNOLOGY(바이오나노학과) > Articles
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