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The spacer arm length in cell-penetrating peptides influences chitosan/siRNA nanoparticle delivery for pulmonary inflammation treatment

Title
The spacer arm length in cell-penetrating peptides influences chitosan/siRNA nanoparticle delivery for pulmonary inflammation treatment
Author
임태연
Keywords
SIRNA DELIVERY; GENE DELIVERY; RNA INTERFERENCE; IN-VITRO; ARGININE; PROGRESS; THERAPY; VECTORS; POLYMER; SYSTEMS
Issue Date
2015-11
Publisher
ROYAL SOC CHEMISTRY
Citation
NANOSCALE, v. 7, NO 47, Page. 20095-20104
Abstract
Although chitosan and its derivatives have been frequently utilized as delivery vehicles for small interfering RNA (siRNA), it is challenging to improve the gene silencing efficiency of chitosan-based nanoparticles. In this study, we hypothesized that controlling the spacer arm length between a cell-penetrating peptide (CPP) and a nanoparticle could be critical to enhancing the cellular uptake as well as the gene silencing efficiency of conventional chitosan/siRNA nanoparticles. A peptide consisting of nine arginine units (R-9) was used as a CPP, and the spacer arm length was controlled by varying the number of glycine units between the peptide (R(9)G(n)) and the nanoparticle (n = 0, 4, and 10). Various physicochemical characteristics of R(9)G(n)-chitosan/siRNA nanoparticles were investigated in vitro. Increasing the spacing arm length did not significantly affect the complex formation between R(9)G(n)-chitosan and siRNA. However, R(9)G(10)-chitosan was much more effective in delivering genes both in vitro and in vivo compared with nonmodified chitosan (without the peptide) and R-9-chitosan (without the spacer arm). Chitosan derivatives modified with oligoarginine containing a spacer arm can be considered as potential delivery vehicles for various genes.
URI
http://pubs.rsc.org/en/Content/ArticleLanding/2015/NR/C5NR06903C#!divAbstracthttp://hdl.handle.net/20.500.11754/28988
ISSN
2040-3364; 2040-3372
DOI
10.1039/c5nr06903c
Appears in Collections:
COLLEGE OF ENGINEERING[S](공과대학) > BIOENGINEERING(생명공학과) > Articles
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