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dc.contributor.author최보율-
dc.date.accessioned2017-09-05T07:32:15Z-
dc.date.available2017-09-05T07:32:15Z-
dc.date.issued2015-11-
dc.identifier.citationONCOTARGET, v. 6, NO 35, Page. 37647-37662en_US
dc.identifier.issn1949-2553-
dc.identifier.urihttp://www.impactjournals.com/oncotarget/index.php?journal=oncotarget&page=article&op=view&path[]=6069&path[]=14841-
dc.identifier.urihttp://hdl.handle.net/20.500.11754/28911-
dc.description.abstractRecent evidence has shown that the level of 5-hydroxymethylcytosine (5hmC) in chromosomal DNA is aberrantly decreased in a variety of cancers, but whether this decrease is a cause or a consequence of tumorigenesis is unclear. Here we show that, in gastric cancers, the 5hmC decrease correlates with a decrease in ten-eleven translocation 1 (TET1) expression, which is strongly associated with metastasis and poor survival in patients with gastric cancer. In gastric cancer cells, TET1-targeted siRNA induced a decrease in 5hmC, whereas TET1 overexpression induced an increase in 5hmC and reduced cell proliferation, thus correlating decreased 5hmC with gastric carcinogenesis. We also report the epigenetic signatures responsible for regulating TET1 transcription. Methyl-CpG Binding Domain Sequencing and Reduced Representation Bisulfite Sequencing identified unique CpG methylation signatures at the CpG island 3'-shore region located 1.3 kb from the transcription start site of TET1 in gastric tumor cells but not in normal mucosa. The luciferase activity of constructs with a methylated 3'-shore sequence was greatly decreased compared with that of an unmethylated sequence in transformed gastric cancer cells. In gastric cancer cells, dense CpG methylation in the 3'-shore was strongly associated with TET1 silencing and bivalent histone marks. Thus, a decrease in 5hmC may be a cause of gastric tumorigenesis owing to a decrease in TET1 expression through DNA methylation coupled with bivalent marks in the 3'-shore of TET1.en_US
dc.description.sponsorshipThis work was supported by the National Research Foundation of Korea (NRF), grants funded by the Korea government (MSIP; no. 2011-0030049 and 2012M3A9B4027954), and a KRIBB Research Initiative Grant.en_US
dc.language.isoenen_US
dc.publisherIMPACT JOURNALS LLCen_US
dc.subjectgastric canceren_US
dc.subjectTET1en_US
dc.subjectDNA methylationen_US
dc.subject5-hydroxymethylcytosineen_US
dc.subject3 '-shoreen_US
dc.subjectbivalent marken_US
dc.titleDecrease of 5hmC in gastric cancers is associated with TET1 silencing due to with DNA methylation and bivalent histone marks at TET1 CpG island 3 '-shoreen_US
dc.typeArticleen_US
dc.relation.no35-
dc.relation.volume6-
dc.identifier.doi10.18632/oncotarget.6069-
dc.relation.page37647-37662-
dc.relation.journalONCOTARGET-
dc.contributor.googleauthorPark, Jong-Lyul-
dc.contributor.googleauthorKim, Hee-Jin-
dc.contributor.googleauthorSeo, Eun-Hye-
dc.contributor.googleauthorKwon, Oh-Hyung-
dc.contributor.googleauthorLim, Byungho-
dc.contributor.googleauthorKim, Mirang-
dc.contributor.googleauthorKim, Seon-Young-
dc.contributor.googleauthorSong, Kyu-Sang-
dc.contributor.googleauthorKang, Gyeong Hoon-
dc.contributor.googleauthorChoi, Bo Youl-
dc.relation.code2015011641-
dc.sector.campusS-
dc.sector.daehakCOLLEGE OF MEDICINE[S]-
dc.sector.departmentDEPARTMENT OF MEDICINE-
dc.identifier.pidbychoi-
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COLLEGE OF MEDICINE[S](의과대학) > MEDICINE(의학과) > Articles
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