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dc.contributor.author박장환-
dc.date.accessioned2017-08-22T06:27:31Z-
dc.date.available2017-08-22T06:27:31Z-
dc.date.issued2015-11-
dc.identifier.citationJOURNAL OF NEUROSCIENCE, v. 35, NO 50, Page. 16479-16493en_US
dc.identifier.issn0270-6474-
dc.identifier.urihttp://www.jneurosci.org/content/35/50/16479-
dc.identifier.urihttp://hdl.handle.net/20.500.11754/28699-
dc.description.abstractExpression of neuregulin-2 (NRG2) is intense in a few regions of the adult brain where neurogenesis persists; however, little is understood about its role in developments of newborn neurons. To study the role of NRG2 in synaptogenesis at different developmental stages, newborn granule cells in rat hippocampal slice cultures were labeled with retrovirus encoding tetracycline-inducible microRNA targeting NRG2 and treated with doxycycline (Dox) at the fourth or seventh postinfection day (dpi). The developmental increase of GABAergic postsynaptic currents (GPSCs) was suppressed by the early Dox treatment (4 dpi), but not by late treatment (7 dpi). The late Dox treatment was used to study the effect of NRG2 depletion specific to excitatory synaptogenesis. The Dox effect on EPSCs emerged 4 d after the impairment in dendritic outgrowth became evident (10 dpi). Notably, Dox treatment abolished the developmental increases of AMPA-receptor mediated EPSCs and the AMPA/NMDA ratio, indicating impaired maturation of glutamatergic synapses. In contrast to GPSCs, Dox effects on EPSCs and dendritic growth were independent of ErbB4 and rescued by concurrent overexpression of NRG2 intracellular domain. These results suggest that forward signaling of NRG2 mediates GABAergic synaptogenesis and its reverse signaling contributes to dendritic outgrowth and maturation of glutamatergic synapses.en_US
dc.description.sponsorshipThis work was supported by the National Research Foundation funded by the Korean Ministry of Science, ICT and Future Planning (Grants 2012-0004904 and 2012-0009135 to S.-H.L. and Grant NRF-2015M3C7A1028790 to W.S.). K.-H.L. is a postdoctorate and C.H.Y. is a graduate student supported by BK21plus.en_US
dc.language.isoenen_US
dc.publisherSOC NEUROSCIENCEen_US
dc.subjectdentate gyrusen_US
dc.subjectErbB4en_US
dc.subjectneuregulinen_US
dc.subjectneurogenesisen_US
dc.subjectsynaptogenesisen_US
dc.titleBidirectional Signaling of Neuregulin-2 Mediates Formation of GABAergic Synapses and Maturation of Glutamatergic Synapses in Newborn Granule Cells of Postnatal Hippocampusen_US
dc.typeArticleen_US
dc.relation.no50-
dc.relation.volume35-
dc.identifier.doi10.1523/JNEUROSCI.1585-15.2015-
dc.relation.page16479-16493-
dc.relation.journalJOURNAL OF NEUROSCIENCE-
dc.contributor.googleauthorLee, Kyu-Hee-
dc.contributor.googleauthorLee, Hyunsu-
dc.contributor.googleauthorYang, Che Ho-
dc.contributor.googleauthorKo, Jeong-Soon-
dc.contributor.googleauthorPark, Chang-Hwan-
dc.contributor.googleauthorWoo, Ran-Sook-
dc.contributor.googleauthorKim, Joo Yeon-
dc.contributor.googleauthorSun, Woong-
dc.contributor.googleauthorKim, Joung-Hun-
dc.contributor.googleauthorHo, Won-Kyung-
dc.relation.code2015000146-
dc.sector.campusS-
dc.sector.daehakCOLLEGE OF MEDICINE[S]-
dc.sector.departmentDEPARTMENT OF MEDICINE-
dc.identifier.pidchshpark-
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COLLEGE OF MEDICINE[S](의과대학) > MEDICINE(의학과) > Articles
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