Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 신흥수 | - |
dc.date.accessioned | 2017-08-09T06:08:42Z | - |
dc.date.available | 2017-08-09T06:08:42Z | - |
dc.date.issued | 2015-10 | - |
dc.identifier.citation | RSC ADVANCES, v. 5, NO 69, Page. 55948-55956 | en_US |
dc.identifier.issn | 2046-2069 | - |
dc.identifier.uri | http://pubs.rsc.org/en/Content/ArticleLanding/2015/RA/C5RA01626F#!divAbstract | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11754/28416 | - |
dc.description.abstract | In this work, we present a biomimetic fibrous scaffold containing two biomolecules. A biocompatible poly(L-lactide-co-epsilon-caprolactone) mesh was fabricated by an electrospinning method, and then acrylic acid was grafted on the mesh to introduce a carboxyl group through gamma-ray irradiation. Subsequently, the epidermal growth factor (EGF) and gelatin were coupled to the mesh through the EDC reaction. The modified mesh presents a consistent fibre diameter (874.4 + 178.5 nm), with carboxyl groups (1.3 mM). EGF (171.7 ng mg(-1) mesh) and gelatin (67.2 +/- 30.5 mu g mg(-1) mesh) were successfully coupled on the mesh. The coupled EGF and gelatin promoted the cell viability 1.5- times higher than that from a non-modified mesh. In particular, the EGF on the meshes independently allowed hMSC to present a 3-times greater involucrin expression and enabled improved procollagen secretion, implying trans-differentiation of hMSC to keratinocyte-like cells. Therefore, the co-immobilization strategy of biomolecules using radiation technology may be an alternative tool for tissue engineering applications. | en_US |
dc.description.sponsorship | This work was supported by National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIP) (2012M2A2A6013196). | en_US |
dc.language.iso | en | en_US |
dc.publisher | ROYAL SOC CHEMISTRY | en_US |
dc.subject | TISSUE ENGINEERING APPLICATIONS | en_US |
dc.subject | EXTRACELLULAR-MATRIX | en_US |
dc.subject | ENDOTHELIAL-CELLS | en_US |
dc.subject | ACRYLIC-ACID | en_US |
dc.subject | SCAFFOLDS | en_US |
dc.subject | PROLIFERATION | en_US |
dc.subject | COLLAGEN | en_US |
dc.subject | FABRICATION | en_US |
dc.subject | DIFFERENTIATION | en_US |
dc.subject | NANOFIBER | en_US |
dc.title | Modulation of human mesenchymal stem cell survival on electrospun mesh with co-immobilized epithelial growth factor and gelatin | en_US |
dc.type | Article | en_US |
dc.relation.no | 69 | - |
dc.relation.volume | 5 | - |
dc.identifier.doi | 10.1039/c5ra01626f | - |
dc.relation.page | 55948-55956 | - |
dc.relation.journal | RSC ADVANCES | - |
dc.contributor.googleauthor | Shin, Young Min | - |
dc.contributor.googleauthor | Lim, Jong-Young | - |
dc.contributor.googleauthor | Park, Jong-Seok | - |
dc.contributor.googleauthor | Gwon, Hui-Jeong | - |
dc.contributor.googleauthor | Jeong, Sung In | - |
dc.contributor.googleauthor | Ahn, Sung-Jun | - |
dc.contributor.googleauthor | Shin, Heungsoo | - |
dc.contributor.googleauthor | Lim, Youn-Mook | - |
dc.relation.code | 2015011569 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF ENGINEERING[S] | - |
dc.sector.department | DEPARTMENT OF BIOENGINEERING | - |
dc.identifier.pid | hshin | - |
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