Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김정목 | - |
dc.date.accessioned | 2017-08-04T01:46:27Z | - |
dc.date.available | 2017-08-04T01:46:27Z | - |
dc.date.issued | 2015-10 | - |
dc.identifier.citation | HELICOBACTER, v. 20, NO 5, Page. 370-380 | en_US |
dc.identifier.issn | 1083-4389 | - |
dc.identifier.issn | 1523-5378 | - |
dc.identifier.uri | http://onlinelibrary.wiley.com/doi/10.1111/hel.12214/abstract;jsessionid=30C5DE153859DC209C5E7FFAE45230CF.f03t02 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11754/28281 | - |
dc.description.abstract | Background: Sequencing of 16S ribosomal RNA (rRNA) gene has improved the characterization of microbial communities. It enabled the detection of low abundance gastric Helicobacter pylori sequences even in subjects that were found to be H. pylori negative with conventional methods. The objective of this study was to obtain a cutoff value for H. pylori colonization in gastric mucosa samples by pyrosequencing method. Materials and Methods: Gastric mucosal biopsies were taken from 63 subjects whose H. pylori status was determined by a combination of serology, rapid urease test, culture, and histology. Microbial DNA from mucosal samples was amplified by PCR using universal bacterial primers. 16S rDNA amplicons were pyrosequenced. ROC curve analysis was performed to determine the cutoff value for H. pylori colonization by pyrosequencing. In addition, temporal changes in the stomach microbiota were observed in eight initially H. pylori-positive and eight H. pylori-negative subjects at a single time point 1-8 years later. Results: Of the 63 subjects, the presence of H. pylori sequences was detected in all (28/28) conventionally H. pylori-positive samples and in 60% (21/35) of H. pylori-negative samples. The average percent of H. pylori reads in each sample was 0.67 +/- 1.09% in the H. pylori-negative group. Cutoff value for clinically positive H. pylori status was approximately 1.22% based on ROC curve analysis (AUC = 0.957; p<.001). Helicobacter pylori was successfully eradicated in five of seven treated H. pylori-positive subjects (71.4%), and the percentage of H. pylori reads in these five subjects dropped from 1.3-95.18% to 0-0.16% after eradication. Conclusion: These results suggest that the cutoff value of H. pylori sequence percentage for H. pylori colonization by pyrosequencing could be set at approximately 1%. It might be helpful to analyze gastric microbiota related to H. pylori sequence status. | en_US |
dc.description.sponsorship | This work was supported by a National Research Foundation (NRF) of Korea grant for the Global Core Research Center (GCRC) funded by the Korea government, Ministry of Science, ICT and Future Planning (MSIP) (No. 2011-0030001). The authors thank the Division of Statistics of the Medical Research Collaborating Center at Seoul National University Bundang Hospital for their assistance in statistical analyses. | en_US |
dc.language.iso | en | en_US |
dc.publisher | WILEY-BLACKWELL | en_US |
dc.subject | 16S ribosomal RNA | en_US |
dc.subject | gastric microbiota | en_US |
dc.subject | Helicobacter pylori | en_US |
dc.subject | stomach | en_US |
dc.title | An Appropriate Cutoff Value for Determining the Colonization of Helicobacter pylori by the Pyrosequencing Method: Comparison with Conventional Methods | en_US |
dc.type | Article | en_US |
dc.relation.no | 5 | - |
dc.relation.volume | 20 | - |
dc.identifier.doi | 10.1111/hel.12214 | - |
dc.relation.page | 370-380 | - |
dc.relation.journal | HELICOBACTER | - |
dc.contributor.googleauthor | Kim, Jaeyeon | - |
dc.contributor.googleauthor | Kim, Nayoung | - |
dc.contributor.googleauthor | Jo, Hyun Jin | - |
dc.contributor.googleauthor | Park, Ji Hyun | - |
dc.contributor.googleauthor | Nam, Ryoung Hee | - |
dc.contributor.googleauthor | Seok, Yeong-Jae | - |
dc.contributor.googleauthor | Kim, Yeon-Ran | - |
dc.contributor.googleauthor | Kim, Joo Sung | - |
dc.contributor.googleauthor | Kim, Jung Mogg | - |
dc.contributor.googleauthor | Kim, Jung Min | - |
dc.relation.code | 2015003415 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF MEDICINE[S] | - |
dc.sector.department | DEPARTMENT OF MEDICINE | - |
dc.identifier.pid | jungmogg | - |
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