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Transplanted Human Amniotic Epithelial Cells Secrete Paracrine Proangiogenic Cytokines in Rat Model of Myocardial Infarction

Title
Transplanted Human Amniotic Epithelial Cells Secrete Paracrine Proangiogenic Cytokines in Rat Model of Myocardial Infarction
Author
신정훈
Keywords
Amniotic epithelial cells; Myocardial infarction (MI); Paracrine; Cytokine
Issue Date
2015-10
Publisher
COGNIZANT COMMUNICATION CORP
Citation
CELL TRANSPLANTATION, v. 24, NO 10, Page. 2055-2064
Abstract
Human amniotic epithelial cells (h-ABCs) have been shown to differentiate into cardiomyocyte-like cells in vivo that can regenerate myocardial tissue and improve cardiac function in a rat model of myocardial infarction (MI). In this study, we investigated the paracrine factors released from h-AECs under hypoxic conditions to elucidate the possible mechanisms underlying this previously reported phenomenon of h-AEC-mediated cardiac repair. We used hypoxic cell culture conditions to simulate myocardial infarction in vitro. In comparison to normal conditions, we found that h-ABCs secreted higher levels of several cytokines, including angiogenin (ANG), epidermal growth factor (EGF), interleukin (IL)-6, and monocyte chemoattractant protein (MCP)-1. To determine whether transplanted h-ABCs express these proangiogenic cytokines in vivo, we ligated the coronary artery of rats to cause MI and injected either h-ABCs or saline into the infarcted area. We found that the infarct and border zones of rat myocardium treated with h-AECs had higher expression levels of the human-origin cytokines ANG, EGF, IL-6, and MCP-1 compared to the tissues of saline-treated rats. In conclusion, h-ABCs secreted proangiogenic cytokines in a rat model of MI, which may suggest that the paracrine effect by h-ABCs could regenerate myocardial tissue and improve cardiac function.
URI
http://journals.sagepub.com/doi/10.3727/096368914X685609http://hdl.handle.net/20.500.11754/28232
ISSN
0963-6897; 1555-3892
DOI
10.3727/096368914X685609
Appears in Collections:
COLLEGE OF MEDICINE[S](의과대학) > MEDICINE(의학과) > Articles
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