Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이상훈 | - |
dc.date.accessioned | 2017-07-31T05:22:31Z | - |
dc.date.available | 2017-07-31T05:22:31Z | - |
dc.date.issued | 2015-10 | - |
dc.identifier.citation | STEM CELL RESEARCH, v. 15, NO 3, Page. 608-613 | en_US |
dc.identifier.issn | 1873-5061 | - |
dc.identifier.issn | 1876-7753 | - |
dc.identifier.uri | http://www.sciencedirect.com/science/article/pii/S1873506115001427?via%3Dihub | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11754/28131 | - |
dc.description.abstract | Culturing human embryonic stem and induced pluripotent stem cells (hESCs/iPSCs) is one of the most costly and labor-intensive tissue cultures, as media containing expensive factors/cytokines should be changed every day to maintain and propagate undifferentiated hESCs/iPSCs in vitro. We recently reported that doxycycline, an antibacterial agent, had dramatic effects on hESC/iPSC survival and promoted self-renewal. In this study, we extended the effects of doxycycline to a more practical issue to save cost and labor in hESC/iPSC cultures. Regardless of cultured cell conditions, hESCs/iPSCs in doxycycline-supplemented media were viable and proliferating for at least 3 days without media change, while none or few viable cells were detected in the absence of doxycycline in the same conditions. Thus, hESCs/iPSCs supplemented with doxycycline can be cultured for a long period of time with media changes at 3-day intervals without altering their self-renewal and pluripotent properties, indicating that doxycycline supplementation can reduce the frequency of media changes and the amount of media required by 1/3. These findings strongly encourage the use of doxycycline to save cost and labor in culturing hESCs/iPSCs. (C) 2015 The Authors. Published by Elsevier B.V. | en_US |
dc.description.sponsorship | This work was supported by a grant from the Medical Research Center (2008-0062190), funded by the National Research Foundation of Korea (NRF) of the Ministry of Education, Science and Technology (MEST), Republic of Korea, and the research fund of Hanyang University (HY-2014). | en_US |
dc.language.iso | en | en_US |
dc.publisher | ELSEVIER SCIENCE BV | en_US |
dc.subject | Human embryonic stem cell | en_US |
dc.subject | Survival | en_US |
dc.subject | Maintenance | en_US |
dc.subject | Doxycycline | en_US |
dc.title | Doxycycline supplementation allows for the culture of human ESCs/iPSCs with media changes at 3-day intervals | en_US |
dc.type | Article | en_US |
dc.relation.no | 3 | - |
dc.relation.volume | 15 | - |
dc.identifier.doi | 10.1016/j.scr.2015.10.007 | - |
dc.relation.page | 608-613 | - |
dc.relation.journal | STEM CELL RESEARCH | - |
dc.contributor.googleauthor | Chang, Mi-Yoon | - |
dc.contributor.googleauthor | Oh, Boram | - |
dc.contributor.googleauthor | Rhee, Yong-Hee | - |
dc.contributor.googleauthor | Lee, Sang-Hun | - |
dc.relation.code | 2015006181 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF MEDICINE[S] | - |
dc.sector.department | DEPARTMENT OF MEDICINE | - |
dc.identifier.pid | leesh | - |
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