247 113

Doxycycline supplementation allows for the culture of human ESCs/iPSCs with media changes at 3-day intervals

Title
Doxycycline supplementation allows for the culture of human ESCs/iPSCs with media changes at 3-day intervals
Author
이상훈
Keywords
Human embryonic stem cell; Survival; Maintenance; Doxycycline
Issue Date
2015-10
Publisher
ELSEVIER SCIENCE BV
Citation
STEM CELL RESEARCH, v. 15, NO 3, Page. 608-613
Abstract
Culturing human embryonic stem and induced pluripotent stem cells (hESCs/iPSCs) is one of the most costly and labor-intensive tissue cultures, as media containing expensive factors/cytokines should be changed every day to maintain and propagate undifferentiated hESCs/iPSCs in vitro. We recently reported that doxycycline, an antibacterial agent, had dramatic effects on hESC/iPSC survival and promoted self-renewal. In this study, we extended the effects of doxycycline to a more practical issue to save cost and labor in hESC/iPSC cultures. Regardless of cultured cell conditions, hESCs/iPSCs in doxycycline-supplemented media were viable and proliferating for at least 3 days without media change, while none or few viable cells were detected in the absence of doxycycline in the same conditions. Thus, hESCs/iPSCs supplemented with doxycycline can be cultured for a long period of time with media changes at 3-day intervals without altering their self-renewal and pluripotent properties, indicating that doxycycline supplementation can reduce the frequency of media changes and the amount of media required by 1/3. These findings strongly encourage the use of doxycycline to save cost and labor in culturing hESCs/iPSCs. (C) 2015 The Authors. Published by Elsevier B.V.
URI
http://www.sciencedirect.com/science/article/pii/S1873506115001427?via%3Dihubhttp://hdl.handle.net/20.500.11754/28131
ISSN
1873-5061; 1876-7753
DOI
10.1016/j.scr.2015.10.007
Appears in Collections:
COLLEGE OF MEDICINE[S](의과대학) > MEDICINE(의학과) > Articles
Files in This Item:
Doxycycline supplementation allows for the culture of human ESCs iPSCs with media changes at 3-day intervals.pdfDownload
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE