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dc.contributor.author윤아름-
dc.date.accessioned2017-07-27T01:19:13Z-
dc.date.available2017-07-27T01:19:13Z-
dc.date.issued2015-10-
dc.identifier.citationONCOTARGET v.6, no.33, Page. 34875-34891en_US
dc.identifier.issn1949-2553-
dc.identifier.urihttp://www.impactjournals.com/oncotarget/index.php?journal=oncotarget&page=article&op=view&path%5b%5d=5332-
dc.identifier.urihttp://hdl.handle.net/20.500.11754/28062-
dc.description.abstractUtility of traditional oncolytic adenovirus (Ad) has been limited due to low expression of coxsackie and adenovirus receptor (CAR) in cancer cells which results in poor infectivity of Ads. Here with an aim of improving the efficiency of Ad's entry to the cell, we generated a novel tropism-expanded oncolytic Ad which contains the epitope of vesicular stomatitis virus glycoprotein (VSVG) at the HI-loop of Ad fiber. We generated 9 variants of oncolytic Ads with varying linkers and partial deletion to the fiber. Only one VSVG epitope-incorporated variant, RdB-1L-VSVG, which contains 1 linker and no deletion to fiber, was produced efficiently. Production of 3-dimensionaly stable fiber in RdB-1L-VSVG was confirmed by immunoblot analysis. RdB-1L-VSVG shows a remarkable improvement in cytotoxicity and total viral yield in cancer cells. RdB-1L-VSVG demonstrates enhanced cytotoxicity in cancer cells with subdued CAR-expression as it can be internalized by an alternate pathway. Competition assays with a CAR-specific antibody (Ab) or VSVG receptor, phosphatidyl serine (PS), reveals that cell internalization of RdB-1L-VSVG is mediated by both CAR and PS. Furthermore, treatment with RdB-1L-VSVG significantly enhanced anti-tumor effect in vivo. These studies demonstrate that the strategy to expand oncolytic Ad tropism may significantly improve therapeutic profile for cancer treatment.en_US
dc.description.sponsorshipThis work was supported by grants from the National Research Foundation of Korea (2010-0029220 and 2013M3A9D3045879).en_US
dc.language.isoenen_US
dc.publisherIMPACT JOURNALS LLCen_US
dc.subjectoncolytic adenovirusen_US
dc.subjectVSVGen_US
dc.subjectfiberen_US
dc.subjectCAR dependencyen_US
dc.subjecttherapeutic efficacyen_US
dc.titleA vesicular stomatitis virus glycoprotein epitope-incorporated oncolytic adenovirus overcomes CAR-dependency and shows markedly enhanced cancer cell killing and suppression of tumor growthen_US
dc.typeArticleen_US
dc.identifier.doi10.18632/oncotarget.5332-
dc.relation.page34875-34891-
dc.relation.journalONCOTARGET-
dc.contributor.googleauthorYoon, A-Rum-
dc.contributor.googleauthorHong, Jinwoo-
dc.contributor.googleauthorYun, Chae-Ok-
dc.relation.code2015011641-
dc.sector.campusS-
dc.sector.daehakCOLLEGE OF ENGINEERING[S]-
dc.sector.departmentDEPARTMENT OF BIOENGINEERING-
dc.identifier.pidayoon-
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COLLEGE OF ENGINEERING[S](공과대학) > BIOENGINEERING(생명공학과) > Articles
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