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dc.contributor.author박홍우-
dc.date.accessioned2017-05-30T00:34:08Z-
dc.date.available2017-05-30T00:34:08Z-
dc.date.issued2015-09-
dc.identifier.citationBIOTECHNOLOGY PROGRESS, v. 31, NO 6, Page. 1536-1541en_US
dc.identifier.issn8756-7938-
dc.identifier.issn1520-6033-
dc.identifier.urihttp://onlinelibrary.wiley.com/doi/10.1002/btpr.2164/full-
dc.identifier.urihttp://hdl.handle.net/20.500.11754/27536-
dc.description.abstractAnalytical chromatography using protein A affinity columns was employed for the fast and simple quantitative analysis of monoclonal antibodies (mAb) from suspension cultures of recombinant Chinese hamster ovary (rCHO) cells. Reliable results could not be obtained from analysis of rCHO cell culture supernatants containing dextran sulfate using elution buffers such as phosphate, glycine, or MgCl2. These problems increased as the number of analysis and the concentration of dextran sulfate in samples increased. Arginine was identified as an alternative eluent to overcome the hindrance by dextran sulfate. When the samples contain dextran sulfate up to 100 mg/L, the elution buffer containing 0.6-1.0 M arginine at pH 3.0-3.8 is useful for the effective analysis. Reproducible results in the mAb quantification could be obtained by this developed arginine elution buffer from rCHO cell culture supernatants containing dextran sulfate. (C) 2015 American Institute of Chemical Engineersen_US
dc.language.isoenen_US
dc.publisherWILEY-BLACKWELLen_US
dc.subjectantibodyen_US
dc.subjectarginineen_US
dc.subjectdextran sulfateen_US
dc.subjectprotein Aen_US
dc.titleArginine as an Eluent Overcomes the Hindrance of Monoclonal Antibody Quantification by Dextran Sulfate in Protein A Affinity Chromatographyen_US
dc.typeArticleen_US
dc.relation.no6-
dc.relation.volume31-
dc.identifier.doi10.1002/btpr.2164-
dc.relation.page1536-1541-
dc.relation.journalBIOTECHNOLOGY PROGRESS-
dc.contributor.googleauthorKim, Bong Gyun-
dc.contributor.googleauthorPark, Hong Woo-
dc.relation.code2015003125-
dc.sector.campusS-
dc.sector.daehakCOLLEGE OF ENGINEERING[S]-
dc.sector.departmentDEPARTMENT OF CHEMICAL ENGINEERING-
dc.identifier.pidparkhw-
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COLLEGE OF ENGINEERING[S](공과대학) > CHEMICAL ENGINEERING(화학공학과) > Articles
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