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Arginine as an Eluent Overcomes the Hindrance of Monoclonal Antibody Quantification by Dextran Sulfate in Protein A Affinity Chromatography

Title
Arginine as an Eluent Overcomes the Hindrance of Monoclonal Antibody Quantification by Dextran Sulfate in Protein A Affinity Chromatography
Author
박홍우
Keywords
antibody; arginine; dextran sulfate; protein A
Issue Date
2015-09
Publisher
WILEY-BLACKWELL
Citation
BIOTECHNOLOGY PROGRESS, v. 31, NO 6, Page. 1536-1541
Abstract
Analytical chromatography using protein A affinity columns was employed for the fast and simple quantitative analysis of monoclonal antibodies (mAb) from suspension cultures of recombinant Chinese hamster ovary (rCHO) cells. Reliable results could not be obtained from analysis of rCHO cell culture supernatants containing dextran sulfate using elution buffers such as phosphate, glycine, or MgCl2. These problems increased as the number of analysis and the concentration of dextran sulfate in samples increased. Arginine was identified as an alternative eluent to overcome the hindrance by dextran sulfate. When the samples contain dextran sulfate up to 100 mg/L, the elution buffer containing 0.6-1.0 M arginine at pH 3.0-3.8 is useful for the effective analysis. Reproducible results in the mAb quantification could be obtained by this developed arginine elution buffer from rCHO cell culture supernatants containing dextran sulfate. (C) 2015 American Institute of Chemical Engineers
URI
http://onlinelibrary.wiley.com/doi/10.1002/btpr.2164/fullhttp://hdl.handle.net/20.500.11754/27536
ISSN
8756-7938; 1520-6033
DOI
10.1002/btpr.2164
Appears in Collections:
COLLEGE OF ENGINEERING[S](공과대학) > CHEMICAL ENGINEERING(화학공학과) > Articles
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