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dc.contributor.author임미선-
dc.date.accessioned2017-05-25T07:38:41Z-
dc.date.available2017-05-25T07:38:41Z-
dc.date.issued2015-09-
dc.identifier.citationPLOS ONE, v. 10, NO 9, Page. 1-14en_US
dc.identifier.issn1932-6203-
dc.identifier.urihttp://journals.plos.org/plosone/article?id=10.1371/journal.pone.0138460-
dc.identifier.urihttp://hdl.handle.net/20.500.11754/27448-
dc.description.abstractDirected methods for differentiating human embryonic stem cells (hESCs) into dopaminergic (DA) precursor cells using stromal cells co-culture systems are already well established. However, not all of the hESCs differentiate into DA precursors using these methods. HSF6, H1, H7, and H9 cells differentiate well into DA precursors, but CHA13 and CHA15 cells hardly differentiate. To overcome this problem, we modified the differentiation system to include a co-culturing step that exposes the cells to noggin early in the differentiation process. This was done using.-irradiated noggin-overexpressing CF1-mouse embryonic fibroblasts (MEF-noggin) and MS5 stromal cells (MS5-noggin and MS5-sonic hedgehog). After directed differentiation, RT-PCR analyses revealed that engrailed-1 (En-1), Lmx1b, and Nurr1, which are midbrain DA markers, were expressed regardless of differentiation stage. Moreover, tyrosine hydroxylase (Th) and an A9 midbrain-specific DA marker (Girk2) were expressed during differentiation, whereas levels of Oct3/4, an undifferentiated marker, decreased. Immunocytochemical analyses revealed that protein levels of the neuronal markers TH and TuJ1 increased during the final differentiation stage. These results demonstrate that early noggin exposure may play a specific role in the directed differentiation of DA cells from human embryonic stem cells.en_US
dc.description.sponsorshipThis work was supported by the Medical Research Center (2008-0062190), the Basic Science Research Program (2011-0008952), and the Bio & Medical Technology Development Program (2012M3A9C7050126) through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT & Future Planning, Hanyang University (201400000000578) and Cooperative Research Program for Agriculture Science & Technology Development (Project No. PJ008022032012) Rural Development Administration, Republic of Korea. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.en_US
dc.language.isoenen_US
dc.publisherPUBLIC LIBRARY SCIENCEen_US
dc.subjectEFFICIENT NEURAL CONVERSIONen_US
dc.subjectIN-VITRO DIFFERENTIATIONen_US
dc.subjectSPEMANN ORGANIZERen_US
dc.subjectPRECURSOR CELLSen_US
dc.subjectMIDBRAINen_US
dc.subjectTRANSPLANTATIONen_US
dc.subjectINHIBITIONen_US
dc.subjectANTAGONISTen_US
dc.subjectACTIVINen_US
dc.subjectDISEASEen_US
dc.titleNoggin Over-Expressing Mouse Embryonic Fibroblasts and MS5 Stromal Cells Enhance Directed Differentiation of Dopaminergic Neurons from Human Embryonic Stem Cellsen_US
dc.typeArticleen_US
dc.relation.no9-
dc.relation.volume10-
dc.identifier.doi10.1371/journal.pone.0138460-
dc.relation.page1-14-
dc.relation.journalPLOS ONE-
dc.contributor.googleauthorLim, Mi-Sun-
dc.contributor.googleauthorShin, Min-Seop-
dc.contributor.googleauthorLee, Soo Young-
dc.contributor.googleauthorMinn, Yang-Ki-
dc.contributor.googleauthorHoh, Jeong-Kyu-
dc.contributor.googleauthorCho, Youl-Hee-
dc.contributor.googleauthorKim, Dong-Wook-
dc.contributor.googleauthorLee, Sang-Hun-
dc.contributor.googleauthorKim, Chun-Hyung-
dc.contributor.googleauthorPark, Chang-Hwan-
dc.relation.code2015008685-
dc.sector.campusS-
dc.sector.daehakRESEARCH INSTITUTE[S]-
dc.sector.departmentHBRI-
dc.identifier.pidmam3128-


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