235 102

Deferoxamine Improves Alveolar and Pulmonary Vascular Development by Upregulating Hypoxia-inducible Factor-1 alpha in a Rat Model of Bronchopulmonary Dysplasia

Title
Deferoxamine Improves Alveolar and Pulmonary Vascular Development by Upregulating Hypoxia-inducible Factor-1 alpha in a Rat Model of Bronchopulmonary Dysplasia
Author
이현주
Keywords
Alveolarization; Bronchopulmonary Dysplasia; Deferoxamine; Hypoxia-Inducible Factor
Issue Date
2015-09
Publisher
KOREAN ACAD MEDICAL SCIENCES
Citation
JOURNAL OF KOREAN MEDICAL SCIENCE, v. 30, NO 9, Page. 1295-1301
Abstract
Fetal lung development normally occurs in a hypoxic environment. Hypoxia-inducible factor (HIF)-1 alpha is robustly induced under hypoxia and transactivates many genes that are essential for fetal development. Most preterm infants are prematurely exposed to hyperoxia, which can halt hypoxia-driven lung maturation. We were to investigate whether the HIF-1 alpha inducer, deferoxamine (DFX) can improve alveolarization in a rat model of bronchopulmonary dysplasia (BPD). A rat model of BPD was produced by intra-amniotic lipopolysaccharide (LPS) administration and postnatal hyperoxia (85% for 7 days), and DFX (150 mg/kg/d) or vehicle was administered to rat pups intraperitoneally for 14 days. On day 14, the rat pups were sacrificed and their lungs were removed and examined. A parallel in vitro study was performed with a human small airway epithelial cell line to test whether DFX induces the expression of HIF-1 alpha and its target genes. Alveolarization and pulmonary vascular development were impaired in rats with BPD. However, DFX significantly ameliorated these effects. Immunohistochemical analysis showed that HIF-1 alpha was significantly upregulated in the lungs of BPD rats treated with DFX. DFX was also found to induce HIF-1 alpha in human small airway epithelial cells and to promote the expression of HIF-1 alpha target genes. Our data suggest that DFX induces and activates HIF-1 alpha, thereby improving alveolarization and vascular distribution in the lungs of rats with BPD.
URI
https://synapse.koreamed.org/search.php?where=aview&id=10.3346/jkms.2015.30.9.1295&code=0063JKMS&vmode=FULLhttp://hdl.handle.net/20.500.11754/27361
ISSN
1011-8934; 1598-6357
DOI
10.3346/jkms.2015.30.9.1295
Appears in Collections:
COLLEGE OF MEDICINE[S](의과대학) > MEDICINE(의학과) > Articles
Files in This Item:
Deferoxamine Improves Alveolar and Pulmonary Vascular Development by Upregulating Hypoxia-inducible Factor-1 alpha in a Rat Model of Bronchopulmona...Download
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE