Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 고성호 | - |
dc.date.accessioned | 2017-04-28T02:06:15Z | - |
dc.date.available | 2017-04-28T02:06:15Z | - |
dc.date.issued | 2015-08 | - |
dc.identifier.citation | MOLECULAR NEUROBIOLOGY, v. 52, NO 1, Page. 792-803 | en_US |
dc.identifier.issn | 0893-7648 | - |
dc.identifier.issn | 1559-1182 | - |
dc.identifier.uri | https://link.springer.com/article/10.1007/s12035-014-8912-5 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11754/27072 | - |
dc.description.abstract | Bone marrow-derived mesenchymal stromal cells (BM-MSCs) represent a promising tool for stem cell-based therapies. However, the majority of MSCs fail to reach the injury site and have only minimal therapeutic effect. In this study, we assessed whether hypoxia/reoxygenation (H/R) preconditioning of human BM-MSCs could increase their functional capacity and beneficial effect on ischemic rat cortical neurons. Human BM-MSCs were cultured under hypoxia (1 % O-2) and with long-term reoxygenation for various times to identify the optimal conditions for increasing their viability and proliferation. The effects of H/R preconditioning on the BM-MSCs were assessed by analyzing the expression of prosurvival genes, trophic factors, and cell migration assays. The functionally improved BM-MSCs were cocultured with ischemic rat cortical neurons to compare with normoxic cultured BM-MSCs. Although the cell viability and proliferation of BM-MSCs were reduced after 1 day of hypoxic culture (1 % O-2), when this was followed by 5-day reoxygenation, the BM-MSCs recovered and multiplied extensively. The immunophenotype and trilineage differentiation of BM-MSCs were also maintained under this H/R preconditioning. In addition, the preconditioning enhanced the expression of prosurvival genes, the messenger RNA (mRNA) levels of various trophic factors and migration capacity. Finally, coculture with the H/R-preconditioned BM-MSCs promoted the survival of ischemic rat cortical neurons. H/R preconditioning of BM-MSCs increases prosurvival signals, trophic factor release, and cell migration and appears to increase their ability to rescue ischemic cortical neurons. This optimized H/R preconditioning procedure could provide the basis for a new strategy for stem cell therapy in ischemic stroke patients. | en_US |
dc.description.sponsorship | This study was supported by a grant of the Korean Health Technology R&D Project, Ministry for Health, Welfare & Family Affairs, Republic of Korea (A101712). | en_US |
dc.language.iso | en | en_US |
dc.publisher | HUMANA PRESS INC | en_US |
dc.subject | Hypoxia/reoxygenation preconditioning | en_US |
dc.subject | Mesenchymal stromal cells | en_US |
dc.subject | Ischemic stroke | en_US |
dc.title | Hypoxia/Reoxygenation-Preconditioned Human Bone Marrow-Derived Mesenchymal Stromal Cells Rescue Ischemic Rat Cortical Neurons by Enhancing Trophic Factor Release | en_US |
dc.type | Article | en_US |
dc.relation.no | 1 | - |
dc.relation.volume | 52 | - |
dc.identifier.doi | 10.1007/s12035-014-8912-5 | - |
dc.relation.page | 792-803 | - |
dc.relation.journal | MOLECULAR NEUROBIOLOGY | - |
dc.contributor.googleauthor | Kim, Young Seo | - |
dc.contributor.googleauthor | Noh, Min Young | - |
dc.contributor.googleauthor | Cho, Kyung Ah | - |
dc.contributor.googleauthor | Kim, Hyemi | - |
dc.contributor.googleauthor | Kwon, Min-Soo | - |
dc.contributor.googleauthor | Kim, Kyung Suk | - |
dc.contributor.googleauthor | Kim, Juhan | - |
dc.contributor.googleauthor | Koh, Seong-Ho | - |
dc.contributor.googleauthor | Kim, Seung Hyun | - |
dc.relation.code | 2015000149 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF MEDICINE[S] | - |
dc.sector.department | DEPARTMENT OF MEDICINE | - |
dc.identifier.pid | ksh213 | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.