Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 류성언 | - |
dc.date.accessioned | 2017-04-24T06:58:08Z | - |
dc.date.available | 2017-04-24T06:58:08Z | - |
dc.date.issued | 2015-08 | - |
dc.identifier.citation | ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, v. 71, Page. 1528-1539 | en_US |
dc.identifier.issn | 2059-7983 | - |
dc.identifier.uri | http://scripts.iucr.org/cgi-bin/paper?dw5138 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11754/26907 | - |
dc.description.abstract | Myotubularin-related proteins are a large family of phosphoinositide phosphatases; their activity, stability and subcellular localization are regulated by dimeric interactions with other members of the family. Here, the crystal structure of the phosphatase domain of MTMR8 is reported. Conformational deviation of the two loops that mediate interaction with the PH-GRAM domain suggests that the PH-GRAM domain interacts differently with the phosphatase domain of each MTMR member. The protein exists as a dimer with twofold symmetry, providing insight into a novel mode of dimerization mediated by the phosphatase domain. Structural comparison and mutation studies suggest that Lys255 of MTMR8 interacts with the substrate diacylglycerol moiety, similar to Lys333 of MTMR2, although the positions of these residues are different. The catalytic activity of the MTMR8 phosphatase domain is inhibited by oxidation and is reversibly reactivated by reduction, suggesting the presence of an oxidation-protective intermediate other than a disulfide bond owing to the absence of a cysteine within a disulfide-bond distance from Cys338. | en_US |
dc.description.sponsorship | K-YY, SJK, SER and Y-SH designed the experiments, K-YY, JYS, JUL, WS and D-WI performed the experiments, K-YY and Y-SH analyzed the data and K-YY, SER and Y-SH wrote the paper. We are grateful to the staff of beamline 7A at Pohang Accelerator Laboratory for help with the diffraction experiments. This work was supported by grants from the National Research Foundation of Korea (NRF-2011-0030027 and NRF-2012R1A1A2008197) funded by the Ministry of Science, ICT and Future Planning. | en_US |
dc.language.iso | en | en_US |
dc.publisher | WILEY-BLACKWELL | en_US |
dc.subject | MTMR8 | en_US |
dc.subject | phosphoinositide | en_US |
dc.subject | phosphatase | en_US |
dc.subject | myotubularin | en_US |
dc.subject | redox regulation | en_US |
dc.title | Structure of the catalytic phosphatase domain of MTMR8: implications for dimerization, membrane association and reversible oxidation | en_US |
dc.type | Article | en_US |
dc.relation.volume | 71 | - |
dc.identifier.doi | 10.1107/S139900471500927X | - |
dc.relation.page | 1528-1539 | - |
dc.relation.journal | ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY | - |
dc.contributor.googleauthor | Yoo, Ki-Young | - |
dc.contributor.googleauthor | Son, Ji Young | - |
dc.contributor.googleauthor | Lee, Jee Un | - |
dc.contributor.googleauthor | Shin, Woori | - |
dc.contributor.googleauthor | Im, Dong-Won | - |
dc.contributor.googleauthor | Kim, Seung Jun | - |
dc.contributor.googleauthor | Ryu, Seong Eon | - |
dc.contributor.googleauthor | Heo, Yong-Seok | - |
dc.relation.code | 2015002240 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF ENGINEERING[S] | - |
dc.sector.department | DEPARTMENT OF BIOENGINEERING | - |
dc.identifier.pid | ryuse | - |
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