167 0

Full metadata record

DC FieldValueLanguage
dc.contributor.author상병인-
dc.date.accessioned2017-04-21T04:56:03Z-
dc.date.available2017-04-21T04:56:03Z-
dc.date.issued2015-08-
dc.identifier.citationBIOPROCESS AND BIOSYSTEMS ENGINEERING, v. 38, NO 11, Page. 2147-2154en_US
dc.identifier.issn1615-7591-
dc.identifier.issn1615-7605-
dc.identifier.urihttps://link.springer.com/article/10.1007/s00449-015-1454-z-
dc.identifier.urihttp://hdl.handle.net/20.500.11754/26870-
dc.description.abstractShewanella oneidensis MR-1 is one of the most well-known metal-reducing bacteria and it has been extensively studied for microbial fuel cell and bioremediation aspects. In this study, we have examined S. oneidensis MR-1 as an isobutanol-producing host by assessing three key factors such as isobutanol synthetic genes, carbon sources, and electron supply systems. Heterologous Ehrlich pathway genes, kivD encoding ketoisovalerate decarboxylase and adh encoding alcohol dehydrogenase, were constructed in S. oneidensis MR-1. Among the composition of carbon sources examined, 2 % of N-acetylglucosamine, 1.5 % of pyruvate and 2 % of lactate were found to be the most optimal nutrients and resulted in 10.3 mg/L of isobutanol production with 48 h of microaerobic incubation. Finally, the effects of metal ions (electron acceptor) and direct electron transfer systems on isobutanol production were investigated, and Fe2+ ions increased the isobutanol production up to 35 %. Interestingly, deletion of mtrA and mtrB, genes responsible for membrane transport systems, did not have significant impact on isobutanol production. Finally, we applied engineered S. oneidensis to a bioelectrical reactor system to investigate the effect of a direct electron supply system on isobutanol production, and it resulted in an increased growth and isobutanol production (up to 19.3 mg/L). This report showed the feasibility of S. oneidensis MR-1 as a genetic host to produce valuable biochemicals and combine an electron-supplying system with biotechnological applications.en_US
dc.description.sponsorshipWe thanks to Prof. Gralnick, Jeffrey A. at BioTechnology Institute and Department of Microbiology, University of Minnesota kindly giving us Delta mtrA and Delta mtrB strains for our study, and Prof. Sinskey at Department of Biology, Massachusetts Institute of Technology for pJL23 plasmid. This work was supported by the R&D Program of MOTIE/KEIT (10048350) and the National Research Foundation of Korea (NRF) (NRF-2013R1A1A2A10004690), (NRF-2015M1A5A1037196). The work was also supported by the Energy Efficiency & Resources of the Korea Institute of Energy Technology Evaluation and Planning (KETEP) grant funded by the Korea Government Ministry of Trade, Industry and Energy (20133030000300) and the Bio & Medical Technology Development Program of the NRF funded by the Korean government, MISP (2015M3A9B8031831).en_US
dc.language.isoenen_US
dc.publisherSPRINGERen_US
dc.subjectIsobutanolen_US
dc.subjectShewanella oneidensis MR-1en_US
dc.subjectElectron transferen_US
dc.titleIsobutanol production from an engineered Shewanella oneidensis MR-1en_US
dc.typeArticleen_US
dc.relation.no11-
dc.relation.volume38-
dc.identifier.doi10.1007/s00449-015-1454-z-
dc.relation.page2147-2154-
dc.relation.journalBIOPROCESS AND BIOSYSTEMS ENGINEERING-
dc.contributor.googleauthorJeon, Jong-Min-
dc.contributor.googleauthorPark, Hyojung-
dc.contributor.googleauthorSeo, Hyung-Min-
dc.contributor.googleauthorKim, Jung-Ho-
dc.contributor.googleauthorBhatia, Shashi Kant-
dc.contributor.googleauthorSathiyanarayanan, Ganesan-
dc.contributor.googleauthorSong, Hun-Suk-
dc.contributor.googleauthorPark, Sung-Hee-
dc.contributor.googleauthorChoi, Kwon-Young-
dc.contributor.googleauthorSang, Byoung-In-
dc.relation.code2015002689-
dc.sector.campusS-
dc.sector.daehakCOLLEGE OF ENGINEERING[S]-
dc.sector.departmentDEPARTMENT OF CHEMICAL ENGINEERING-
dc.identifier.pidbiosang-
Appears in Collections:
COLLEGE OF ENGINEERING[S](공과대학) > CHEMICAL ENGINEERING(화학공학과) > Articles
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE