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Quantification of Ciprofloxacin in Human Plasma by Validated LC-MS/MS Using Ofloxacin as an Internal Standard and its Clinical Application

Title
Quantification of Ciprofloxacin in Human Plasma by Validated LC-MS/MS Using Ofloxacin as an Internal Standard and its Clinical Application
Author
박유신
Keywords
Bioequivalence; ciprofloxacin; LC-MS/MS; liquid-liquid extraction; pharmacokinetics; internal standard
Issue Date
2015-07
Publisher
BENTHAM SCIENCE PUBL LTD
Citation
CURRENT PHARMACEUTICAL ANALYSIS, v. 11, NO 4, Page. 239-247
Abstract
A simple LC-MS/MS method has been advanced for measuring ciprofloxacin, using liquid-liquid extraction of plasma with ether and methylene chloride (7/3, v/v) using ofloxacin as an internal standard (IS). The analytes were detected by HPLC using an isocratic mobile phase composed of methanol and 5 mM aqueous ammonium acetate (pH 3.0, 35/65, v/v) on a Luna phenyl-hexyl (Phenomenex 2.0 x 50 mm, 5 mu m) column. The eluate was analyzed by MS/MS in the MRM mode using the transitions of the respective (M+H)(+) ions (m/z 332.1 -˃ 231.1 and m/z 362.1 -˃ 261.1) for determination of ciprofloxacin and IS, respectively. The standard calibration curves indicated good linearity within the range of 0.05-5.0 mu g/ml (r(2) = 0.997, 1/x(2) weighting). The lower limit of quantification (LLOQ) was 0.05 mu g/ml. The retention times of ciprofloxacin and IS were 1.79 and 1.51 minutes, respectively. In addition, no significant impurities were found that interfered with the analysis. Acceptable precision and accuracy were obtained for the concentration range of the standard curve. The validated method was successfully applied to bioequivalence and pharmacokinetic studies of two formulations of ciprofloxacin (single 250 mg doses) in 24 healthy Korean volunteers.
URI
http://www.ingentaconnect.com/content/ben/cpa/2015/00000011/00000004/art00004http://hdl.handle.net/20.500.11754/26540
ISSN
1573-4129; 1875-676X
DOI
10.2174/1573412911666150331224024
Appears in Collections:
COLLEGE OF HUMAN ECOLOGY[S](생활과학대학) > FOOD & NUTRITION(식품영양학과) > Articles
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