473 0

감마선을 이용한 아크릴산이 도입된 골조직공학용 PCL/BCP 나노섬유 지지체의 개발

Title
감마선을 이용한 아크릴산이 도입된 골조직공학용 PCL/BCP 나노섬유 지지체의 개발
Other Titles
Development of Acrylic Acid Grafted Polycaprolactone (PCL)/Biphasic Calcium Phosphate (BCP) Nanofibers for Bone Tissue Engineering Using Gamma-Irradiation
Author
신흥수
Keywords
polycaprolactone; biphasic calcium phosphate; electrospinning; gamma-irradiation; acrylic acid; bone tissue engineering
Issue Date
2015-07
Publisher
POLYMER SOC KOREA
Citation
POLYMER-KOREA, v. 39, NO 3, Page. 418-425
Abstract
Polycaprolactone (PCL)과 biphasic calcium phosphate (BCP)는 생체적합성 및 골 형성 촉진 등으로 인해 정형외과 소재로 사용되고 있다. 하지만,PCL은 표면이 소수성으로 인해 세포의 부착 및 증식에 제한적이기 때문에 이를 극복하기 위해 본 연구에서는 감마선을 이용하여 골 재생을 위한 친수성이 향상된 PLC/BCP 나노섬유를 제조하였다.나노섬유는 전기방사를 통해 제작되었으며, 감마선을 이용하여 acrylic acid (AAc)를 도입하였다. SEM을 통해 나노 섬유 표면을 확인하였고, AAc가 도입된 나노섬유를 위해서 MG63의 초기 생존율이 현저히 증가한 것을 확인하였다. 알칼리성 포스파테이즈 활성은 1.239 +/- 0.226 nmole/mu g/min으로 개질되지 않은 나노섬유(0.590 +/- 0.286 nmole/mu g/min)보다 증가하였다.따라서 AAc가 도입된 PCL/BCP 나노섬유는 골조직 재생에 활용될 수 있을 것으로 기대된다. Polycaprolactone (PCL) and biphasic calcium phosphate (BCP) have been considered as useful materials for orthopedic devices and osseous implants because of their biocompatibility and bone-forming activity. However, PCL-based scaffolds have hydrophobic surfaces reducing initial cell adhesion or proliferation. To overcome the limitation, we fabricated surface-modified PCL/BCP nanofibers using gamma-irradiation for bone tissue engineering. PCL/BCP nanofibers were prepared by electrospinning and then we supplemented hydrophilicity by introducing acrylic acid (AAc) through gamma-irradiation. We confirmed the surface of nanofibers by SEM, and then the initial viability of MG63 was significantly increased on the AAc grafted nanofibers, and alkaline phosphatase activity(1.239 +/- 0.226 nmole/mu g/min) improved on the modified nanofibers than that on the non-modified nanofibers(0.590 +/- 0.286 nmole/mu g/min). Therefore, AAc-grafted nanofibers may be a good tool for bone tissue engineering applications.
URI
http://www.koreascience.or.kr/article/ArticleFullRecord.jsp?cn=GBJHCY_2015_v39n3_418http://hdl.handle.net/20.500.11754/26344
ISSN
0379-153X; 2234-8077
DOI
10.7317/pk.2015.39.3.418
Appears in Collections:
COLLEGE OF ENGINEERING[S](공과대학) > BIOENGINEERING(생명공학과) > Articles
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE