Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김진웅 | - |
dc.date.accessioned | 2017-03-23T07:22:17Z | - |
dc.date.available | 2017-03-23T07:22:17Z | - |
dc.date.issued | 2015-07 | - |
dc.identifier.citation | NANO LETTERS, v. 15, NO 7, Page. 4737-4744 | en_US |
dc.identifier.issn | 1530-6984 | - |
dc.identifier.issn | 1530-6992 | - |
dc.identifier.uri | http://pubs.acs.org/doi/abs/10.1021/acs.nanolett.5b01636 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11754/26293 | - |
dc.description.abstract | Recent development of liquid phase transmission electron microscopy (TEM) enables the study of specimens in wet ambient conditions within a liquid cell; however, direct structural observation of biological samples in their native solution using TEM is challenging since low-mass biomaterials embedded in a thick liquid layer of the host cell demonstrate low contrast. Furthermore, the integrity of delicate wet samples is easily compromised during typical sample preparation and TEM imaging. To overcome these limitations, we introduce a graphene liquid cell (GLC) using multilayer graphene sheets to reliably encapsulate and preserve biological samples in a liquid for TEM observation. We achieve nanometer scale spatial resolution with high contrast using low-dose TEM at room temperature, and we use the GLC to directly observe the structure of influenza viruses in their native buffer solution at room temperature. The GLC is further extended to investigate whole cells in wet conditions using TEM. We also demonstrate the potential of the GLC for correlative studies by TEM and fluorescence light microscopy imaging. | en_US |
dc.description.sponsorship | J.P, S.H.H, and D.A.W. acknowledge support from Amore Pacific. This work is also supported by the Harvard Materials Research Science and Engineering Center (DMR-1420570). The aberration-corrected electron microscopy was performed at the Molecular Foundry supported by the US Department of Energy DE-AC02-05CH11231. J.W.K. acknowledges support from the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIP) (No. 2008-0061891). | en_US |
dc.language.iso | en | en_US |
dc.publisher | AMER CHEMICAL SOC | en_US |
dc.subject | Liquid phase TEM | en_US |
dc.subject | graphene liquid cell | en_US |
dc.subject | multilayer graphene | en_US |
dc.subject | bioimaging | en_US |
dc.subject | influenza viruses | en_US |
dc.subject | epithelial cells | en_US |
dc.title | Direct Observation of Wet Biological Samples by Graphene Liquid Cell Transmission Electron Microscopy | en_US |
dc.type | Article | en_US |
dc.relation.no | 7 | - |
dc.relation.volume | 15 | - |
dc.identifier.doi | 10.1021/acs.nanolett.5b01636 | - |
dc.relation.page | 4737-4744 | - |
dc.relation.journal | NANO LETTERS | - |
dc.contributor.googleauthor | Park, Jungwon | - |
dc.contributor.googleauthor | Park, Hyesung | - |
dc.contributor.googleauthor | Ercius, Peter | - |
dc.contributor.googleauthor | Pegoraro, Adrian F. | - |
dc.contributor.googleauthor | Xu, Chen | - |
dc.contributor.googleauthor | Kim, Jin Woong | - |
dc.contributor.googleauthor | Han, Sang Hoon | - |
dc.contributor.googleauthor | Weitz, David A. | - |
dc.relation.code | 2015000565 | - |
dc.sector.campus | S | - |
dc.sector.daehak | GRADUATE SCHOOL[S] | - |
dc.sector.department | DEPARTMENT OF BIONANOTECHNOLOGY | - |
dc.identifier.pid | kjwoong | - |
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