238 0

Full metadata record

DC FieldValueLanguage
dc.contributor.author김진혁-
dc.date.accessioned2017-03-20T01:02:56Z-
dc.date.available2017-03-20T01:02:56Z-
dc.date.issued2015-07-
dc.identifier.citationJOURNAL OF BIOLOGICAL CHEMISTRY, v. 290, NO 28, Page. 17401-17414en_US
dc.identifier.issn0021-9258-
dc.identifier.issn1083-351X-
dc.identifier.urihttp://www.jbc.org/content/290/28/17401.short-
dc.identifier.urihttp://hdl.handle.net/20.500.11754/26201-
dc.description.abstractRecent groundbreaking work has demonstrated that combined expression of the transcription factors Brn2, Ascl1, and Myt1L (BAM; also known as Wernig factors) convert mouse fibroblasts into postmitotic neuronal cells. However, questions remain regarding whether trans-conversion is achieved directly or involves an intermediary precursor stage. Trans-conversion toward expandable neural precursor cells (NPCs) is more useful than direct one-step neuron formation with respect to yielding a sufficient number of cells and the feasibility of manipulating NPC differentiation toward certain neuron subtypes. Here, we show that co-expression of Wernig factors and Bcl-xL induces fibroblast conversion into NPCs (induced NPCs (iNPCs)) that are highly expandable for > 100 passages. Gene expression analyses showed that the iNPCs exhibited high expression of common NPC genes but not genes specific to defined embryonic brain regions. This finding indicated that a regional identity of iNPCs was not established. Upon induction, iNPCs predominantly differentiated into astrocytes. However, the differentiation potential was not fixed and could be efficiently manipulated into general or specific subtypes of neurons by expression of additional genes. Specifically, overexpression of Nurr1 and Foxa2, transcription factors specific for midbrain dopamine neuron development, drove iNPCs to yield mature midbrain dopamine neurons equipped with presynaptic DA neuronal functions. We further assessed the therapeutic potential of iNPCs in Parkinson disease model rats.en_US
dc.description.sponsorshipThis work was supported by Medical Research Center Grant 2008-0062190; Basic Science Research Program Grant 2011-0008952; Bio & Medical Technology Development Program Grants 2010-0020232 and 2012 M3A9C7050126; and Korea National Research Council of Science and Technology Program Grant NAP-09-04 through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT, and Future Planning.en_US
dc.language.isoenen_US
dc.publisherAMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INCen_US
dc.subjectEMBRYONIC STEM-CELLSen_US
dc.subjectIN-VITROen_US
dc.subjectMOUSE FIBROBLASTSen_US
dc.subjectDIRECT CONVERSIONen_US
dc.subjectMICROGLIAL ACTIVATIONen_US
dc.subjectGENE-EXPRESSIONen_US
dc.subjectDEFINED FACTORSen_US
dc.subjectSOMATIC-CELLSen_US
dc.subjectDIFFERENTIATIONen_US
dc.subjectISCHEMIAen_US
dc.titleGeneration of Dopamine Neurons from Rodent Fibroblasts through the Expandable Neural Precursor Cell Stageen_US
dc.typeArticleen_US
dc.relation.no28-
dc.relation.volume290-
dc.identifier.doi10.1074/jbc.M114.629808-
dc.relation.page17401-17414-
dc.relation.journalJOURNAL OF BIOLOGICAL CHEMISTRY-
dc.contributor.googleauthorLim, Mi-Sun-
dc.contributor.googleauthorChang, Mi-Yoon-
dc.contributor.googleauthorKim, Sang-Mi-
dc.contributor.googleauthorYi, Sang-Hoon-
dc.contributor.googleauthorSuh-Kim, Haeyoung-
dc.contributor.googleauthorJung, Sung Jun-
dc.contributor.googleauthorKim, Min Jung-
dc.contributor.googleauthorKim, Jin Hyuk-
dc.contributor.googleauthorLee, Yong-Sung-
dc.contributor.googleauthorLee, Soo Young-
dc.relation.code2015002335-
dc.sector.campusS-
dc.sector.daehakCOLLEGE OF MEDICINE[S]-
dc.sector.departmentDEPARTMENT OF MEDICINE-
dc.identifier.pidjhkim1-
Appears in Collections:
COLLEGE OF MEDICINE[S](의과대학) > MEDICINE(의학과) > Articles
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE