Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이민형 | - |
dc.date.accessioned | 2017-03-17T00:43:32Z | - |
dc.date.available | 2017-03-17T00:43:32Z | - |
dc.date.issued | 2015-07 | - |
dc.identifier.citation | YONSEI MEDICAL JOURNAL, v. 56, NO 4, Page. 1036-1043 | en_US |
dc.identifier.issn | 0513-5796 | - |
dc.identifier.issn | 1976-2437 | - |
dc.identifier.uri | https://synapse.koreamed.org/DOIx.php?id=10.3349/ymj.2015.56.4.1036 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11754/26167 | - |
dc.description.abstract | Purpose: Spinal cord injury (SCI) is associated with permanent neurological damage, and treatment thereof with a single modality often does not provide sufficient therapeutic outcomes. Therefore, a strategy that combines two or more techniques might show better therapeutic effects. Materials and Methods: In this study, we designed a combined treatment strategy based on neural stem cells (NSCs) introduced via a neuronal cell type inducible transgene expression system (NSE::) controlled by a neuron-specific enolase (NSE) promoter to maximize therapeutic efficiency and neuronal differentiation. The luciferase gene was chosen to confirm whether this combined system was working properly prior to using a therapeutic gene. The luciferase expression levels of NSCs introduced via the neuronal cell type inducible luciferase expression system (NSE::Luci) or via a general luciferase expressing system (SV::Luci) were measured and compared in vitro and. in vivo. Results: NSCs introduced via the neuronal cell type inducible luciferase expressing system (NSE::Luci-NSCs) showed a high level of luciferase expression, compared to NSCs introduced via a general luciferase expressing system (SV::Luci-NSCs). Interestingly, the luciferase expression level of NSE::Luci-NSCs increased greatly after differentiation into neurons. Conclusion: We demonstrated that a neuronal cell type inducible gene expression system is suitable for introducing NSCs in combined treatment strategies. We suggest that the proposed strategy may be a promising tool for the treatment of neurodegenerative disorders, including SCI. | en_US |
dc.description.sponsorship | This study was supported by a grant from the Korea Health Technology R&D Project, Ministry of Health & Welfare, Republic of Korea (A120254); a National Research Foundation of Korea Grant funded by the Korean Government (2012-0001560); and faculty research grants from Yonsei University College of Medicine for 2014 (No. 6-2014-0035). | en_US |
dc.language.iso | en | en_US |
dc.publisher | YONSEI UNIV COLL MEDICINE | en_US |
dc.subject | Neuronal cell type-inducible transgene expression | en_US |
dc.subject | neural stem cells | en_US |
dc.subject | combined treatment strategy | en_US |
dc.subject | spinal cord injury | en_US |
dc.title | A Gene and Neural Stem Cell Therapy Platform Based on Neuronal Cell Type-Inducible Gene Overexpression | en_US |
dc.type | Article | en_US |
dc.relation.no | 4 | - |
dc.relation.volume | 56 | - |
dc.identifier.doi | 10.3349/ymj.2015.56.4.1036 | - |
dc.relation.page | 1036-1043 | - |
dc.relation.journal | YONSEI MEDICAL JOURNAL | - |
dc.contributor.googleauthor | Oh, Jinsoo | - |
dc.contributor.googleauthor | You, Youngsang | - |
dc.contributor.googleauthor | Yun, Yeomin | - |
dc.contributor.googleauthor | Lee, Hye-Lan | - |
dc.contributor.googleauthor | Yoon, Do Heum | - |
dc.contributor.googleauthor | Lee, Minhyung | - |
dc.contributor.googleauthor | Ha, Yoon | - |
dc.relation.code | 2015000736 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF ENGINEERING[S] | - |
dc.sector.department | DEPARTMENT OF BIOENGINEERING | - |
dc.identifier.pid | minhyung | - |
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