Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김영필 | - |
dc.date.accessioned | 2017-03-13T07:07:01Z | - |
dc.date.available | 2017-03-13T07:07:01Z | - |
dc.date.issued | 2015-07 | - |
dc.identifier.citation | SENSORS, v. 15, NO 8, Page. 17977-17989 | en_US |
dc.identifier.issn | 1424-8220 | - |
dc.identifier.uri | http://www.mdpi.com/1424-8220/15/8/17977/htm | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11754/26064 | - |
dc.description.abstract | We report a simple detection of protein kinase activity using Zn(II)-mediated fluorescent resonance energy transfer (FRET) between quantum dots (QDs) and dye-tethered peptides. With neither complex chemical ligands nor surface modification of QDs, Zn(II) was the only metal ion that enabled the phosphorylated peptides to be strongly attached on the carboxyl groups of the QD surface via metal coordination, thus leading to a significant FRET efficiency. As a result, protein kinase activity in intermixed solution was efficiently detected by QD-FRET via Zn(II) coordination, especially when the peptide substrate was combined with affinity-based purification. We also found that mono- and di-phosphorylation in the peptide substrate could be discriminated by the Zn(II)-mediated QD-FRET. Our approach is expected to find applications for studying physiological function and signal transduction with respect to protein kinase activity. | en_US |
dc.description.sponsorship | This work was supported by Mid-career Researcher Program (No. 2013R1A2A2A03015161 to Y.-P.K.) and Nano-Material Technology Development Program (No. 2012M3A7B4035286 to Y.-P.K.) through the National Research Foundation (NRF) funded by the Ministry of Science, ICT, and Future Planning (MSIP). This work was also supported by grants from the Next-Generation BioGreen 21 Program (SSAC, PJ01114901 to T.W.K), Rural Development Administration, Korea. | en_US |
dc.language.iso | en | en_US |
dc.publisher | MDPI AG | en_US |
dc.subject | FRET | en_US |
dc.subject | quantum dot | en_US |
dc.subject | zinc | en_US |
dc.subject | kinase | en_US |
dc.subject | phosphorylation | en_US |
dc.title | Zn(II)-Coordinated Quantum Dot-FRET Nanosensors for the Detection of Protein Kinase Activity | en_US |
dc.type | Article | en_US |
dc.relation.no | 8 | - |
dc.relation.volume | 15 | - |
dc.identifier.doi | 10.3390/s150817977 | - |
dc.relation.page | 17977-17989 | - |
dc.relation.journal | SENSORS | - |
dc.contributor.googleauthor | Lim, Butaek | - |
dc.contributor.googleauthor | Park, Ji-In | - |
dc.contributor.googleauthor | Lee, Kyung Jin | - |
dc.contributor.googleauthor | Lee, Jin-Won | - |
dc.contributor.googleauthor | Kim, Tae-Wuk | - |
dc.contributor.googleauthor | Kim, Young-Pil | - |
dc.relation.code | 2015009172 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF NATURAL SCIENCES[S] | - |
dc.sector.department | DEPARTMENT OF LIFE SCIENCE | - |
dc.identifier.pid | ypilkim | - |
dc.identifier.researcherID | A-5437-2009 | - |
dc.identifier.orcid | http://orcid.org/0000-0001-7234-1320 | - |
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