Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jong Wook Hong | - |
dc.date.accessioned | 2017-03-08T04:49:55Z | - |
dc.date.available | 2017-03-08T04:49:55Z | - |
dc.date.issued | 2015-06 | - |
dc.identifier.citation | JOURNAL OF HAZARDOUS MATERIALS, v. 298, Page. 188-194 | en_US |
dc.identifier.issn | 0304-3894 | - |
dc.identifier.issn | 1873-3336 | - |
dc.identifier.uri | http://www.sciencedirect.com/science/article/pii/S0304389415004379 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11754/25939 | - |
dc.description.abstract | Francisella tularensis is a human zoonotic pathogen and the causative agent of tularemia, a severe infectious disease. Given the extreme infectivity of F. tularensis and its potential to be used as a biological warfare agent, a fast and sensitive detection method is highly desirable. Herein, we construct a novel detection platform composed of two units: (I) Magnetic beads conjugated with multiple capturing antibodies against F. tularensis for its simple and rapid separation and (2) Genetically-engineered apoferritin protein constructs conjugated with multiple quantum dots and a detection antibody against F. tularensis for the amplification of signal. We demonstrate a 10-fold increase in the sensitivity relative to traditional lateral flow devices that utilize enzyme-based detection methods. We ultimately envision the use of our novel nanoprobe detection platform in future applications that require the highly-sensitive on-site detection of high-risk pathogens. (C) 2015 Elsevier B.V. All rights reserved. | en_US |
dc.description.sponsorship | This work was supported by the Basic Science Research Program through the National Research Foundation (NRF) funded by the Ministry of Education (grant number: 2013R1A1A1012653), by the Korea Centers for Disease Control and Prevention (grant number: 2014-E45002-00) and the grant of the Korea Health Technology R&D Project through the Korea Health Industry Development Institute(KHIDI), funded by the Ministry of Health & Welfare, Republic of Korea (grant number: HI14C3266). | en_US |
dc.language.iso | en | en_US |
dc.publisher | ELSEVIER SCIENCE BV | en_US |
dc.subject | Nanoprobes | en_US |
dc.subject | Francisella tularensis | en_US |
dc.subject | Apoferritin | en_US |
dc.subject | Immunoassay | en_US |
dc.subject | Biosensing | en_US |
dc.title | A novel nanoprobe for the sensitive detection of Francisella tularensis | en_US |
dc.type | Article | en_US |
dc.relation.volume | 298 | - |
dc.identifier.doi | 10.1016/j.jhazmat.2015.05.041 | - |
dc.relation.page | 188-194 | - |
dc.relation.journal | JOURNAL OF HAZARDOUS MATERIALS | - |
dc.contributor.googleauthor | Kim, Ji-eun | - |
dc.contributor.googleauthor | Seo, Youngmin | - |
dc.contributor.googleauthor | Jeong, Yoon | - |
dc.contributor.googleauthor | Hwang, Mintai P. | - |
dc.contributor.googleauthor | Hwang, Jangsun | - |
dc.contributor.googleauthor | Choo, Jaeburn | - |
dc.contributor.googleauthor | Hong, Jong Wook | - |
dc.contributor.googleauthor | Jeon, Jun Ho | - |
dc.contributor.googleauthor | Rhie, Gi-eun | - |
dc.contributor.googleauthor | Choi, Jonghoon | - |
dc.relation.code | 2015000315 | - |
dc.sector.campus | S | - |
dc.sector.daehak | GRADUATE SCHOOL[S] | - |
dc.sector.department | DEPARTMENT OF BIONANOTECHNOLOGY | - |
dc.identifier.pid | jwh | - |
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