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dc.contributor.author윤문영-
dc.date.accessioned2017-03-02T04:25:57Z-
dc.date.available2017-03-02T04:25:57Z-
dc.date.issued2015-06-
dc.identifier.citationINTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, v. 77, Page. 293-302en_US
dc.identifier.issn0141-8130-
dc.identifier.issn1879-0003-
dc.identifier.urihttp://www.sciencedirect.com/science/article/pii/S0141813015001956-
dc.identifier.urihttp://hdl.handle.net/20.500.11754/25742-
dc.description.abstractAnthrax toxin excreted by Bacillus anthracis is the key causative agent of infectious anthrax disease. In the present study, we targeted the binding of PA to the ATR/TEM8 Von Willebrand factor type A (VWA) domain, which we cloned into Escherichia colt and purified to homogeneity under denaturing conditions. To develop an anthrax toxin inhibitor, we selected and identified short single strand RNA aptamers (approximately 30mer) consisting of different sequences of nucleic acids with a high binding affinity in the 100 nanomolar range against the recombinant ATR/TEM8 VWA domain using systematic evolution of ligands by exponential enrichment (SELEX). Five candidate aptamers were further characterized by several techniques including secondary structural analysis. The inhibitor efficiency (IC50) of one of the aptamers toward anthrax toxin was approximately 5 mu M in macrophage RAW 264.7 cells, as determined from cytotoxicity analysis by MU assay. We believe that the candidate aptamers should be useful for blocking the binding of PA to its receptor in order to neutralize anthrax toxin. (C) 2015 Elsevier B.V. All rights reserved.en_US
dc.description.sponsorshipThis work was supported by the Converging Research Center Program, Ministry of Science, ICT & Future Planning, Republic of Korea (Project No. 2014048814) and by the Bio-Industry Technology Development Program, Ministry of Food, Agriculture, Forestry and Fisheries, Republic of Korea (Project No. 111079-3).en_US
dc.language.isoenen_US
dc.publisherELSEVIER SCIENCE BVen_US
dc.subjectAnthrax toxinen_US
dc.subjectATRen_US
dc.subjectRNA aptameren_US
dc.titleDevelopment of receptor-based inhibitory RNA aptamers for anthrax toxin neutralizationen_US
dc.typeArticleen_US
dc.relation.volume77-
dc.identifier.doi10.1016/j.ijbiomac.2015.03.043-
dc.relation.page293-302-
dc.relation.journalINTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES-
dc.contributor.googleauthorLee, Sang-Choon-
dc.contributor.googleauthorGedi, Vinayakumar-
dc.contributor.googleauthorHa, Na-Reum-
dc.contributor.googleauthorCho, Jun-Haeng-
dc.contributor.googleauthorPark, Hae-Chul-
dc.contributor.googleauthorYoon, Moon-Young-
dc.relation.code2015001387-
dc.sector.campusS-
dc.sector.daehakCOLLEGE OF NATURAL SCIENCES[S]-
dc.sector.departmentDEPARTMENT OF CHEMISTRY-
dc.identifier.pidmyyoon-
Appears in Collections:
COLLEGE OF NATURAL SCIENCES[S](자연과학대학) > CHEMISTRY(화학과) > Articles
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