Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김계성 | - |
dc.date.accessioned | 2017-02-22T02:13:26Z | - |
dc.date.available | 2017-02-22T02:13:26Z | - |
dc.date.issued | 2015-06 | - |
dc.identifier.citation | CELL, v. 161, NO 7, Page. 1553-1565 | en_US |
dc.identifier.issn | 0092-8674 | - |
dc.identifier.issn | 1097-4172 | - |
dc.identifier.uri | http://www.sciencedirect.com/science/article/pii/S0092867415005747 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11754/25608 | - |
dc.description.abstract | Hematopoietic stem cells (HSCs) reside in hypoxic niches within bone marrow and cord blood. Yet, essentially all HSC studies have been performed with cells isolated and processed in non-physiologic ambient air. By collecting and manipulating bone marrow and cord blood in native conditions of hypoxia, we demonstrate that brief exposure to ambient oxygen decreases recovery of long-term repopulating HSCs and increases progenitor cells, a phenomenon we term extraphysiologic oxygen shock/stress (EPHOSS). Thus, true numbers of HSCs in the bone marrow and cord blood are routinely underestimated. We linked ROS production and induction of the mitochondrial permeability transition pore (MPTP) via cyclophilin D and p53 as mechanisms of EPHOSS. The MPTP inhibitor cyclosporin A protects mouse bone marrow and human cord blood HSCs from EPHOSS during collection in air, resulting in increased recovery of transplantable HSCs. Mitigating EPHOSS during cell collection and processing by pharmacological means may be clinically advantageous for transplantation. | en_US |
dc.description.sponsorship | Research was supported by US Public Health Service Grants from the NIH (R01 HL67384, R01 HL056416, R01 HL112669, and P30 DK090948 to H.E.B.), the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT & Future Planning (2012M3A9B4028738), and a grant of the Korea Health technology R&D Project, Ministry of Health & Welfare (A120262), Republic of Korea (to K.-S.K.). H.A.O. and S.M-G. were supported by NIH T32 training grant DK07519 (to H.E.B.). N. Brustovetsky was supported by NIH grant R01 NS078008. We thank P.L. Mantel for editorial assistance. We would like to dedicate this paper to Dr. Donald Metcalf, a pioneer in the field of hematopoiesis, who recently passed away. | en_US |
dc.language.iso | en | en_US |
dc.publisher | CELL PRESS | en_US |
dc.subject | MITOCHONDRIAL PERMEABILITY TRANSITION | en_US |
dc.subject | ADENINE-NUCLEOTIDE TRANSLOCASE | en_US |
dc.subject | UMBILICAL-CORD BLOOD | en_US |
dc.subject | CYCLOSPORINE-A | en_US |
dc.subject | OXIDATIVE STRESS | en_US |
dc.subject | PROGENITOR CELLS | en_US |
dc.subject | BONE-MARROW | en_US |
dc.subject | INNER-MEMBRANE | en_US |
dc.subject | CYCLOPHILIN-D | en_US |
dc.subject | ISCHEMIA/REPERFUSION INJURY | en_US |
dc.title | Enhancing Hematopoietic Stem Cell Transplantation Efficacy by Mitigating Oxygen Shock | en_US |
dc.type | Article | en_US |
dc.relation.no | 7 | - |
dc.relation.volume | 161 | - |
dc.identifier.doi | 10.1016/j.cell.2015.04.054 | - |
dc.relation.page | 1553-1565 | - |
dc.relation.journal | CELL | - |
dc.contributor.googleauthor | Mantel, Charlie R. | - |
dc.contributor.googleauthor | O'Leary, Heather A. | - |
dc.contributor.googleauthor | Chitteti, Brahmananda R. | - |
dc.contributor.googleauthor | Huang, XinXin | - |
dc.contributor.googleauthor | Cooper, Scott | - |
dc.contributor.googleauthor | Hangoc, Giao | - |
dc.contributor.googleauthor | Brustovetsky, Nickolay | - |
dc.contributor.googleauthor | Srour, Edward F. | - |
dc.contributor.googleauthor | Lee, Man Ryul | - |
dc.contributor.googleauthor | Kim, Kye-Seong | - |
dc.relation.code | 2015017312 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF MEDICINE[S] | - |
dc.sector.department | DEPARTMENT OF MEDICINE | - |
dc.identifier.pid | ks66kim | - |
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