Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김계성 | - |
dc.date.accessioned | 2017-02-16T05:57:40Z | - |
dc.date.available | 2017-02-16T05:57:40Z | - |
dc.date.issued | 2015-06 | - |
dc.identifier.citation | TISSUE ENGINEERING AND REGENERATIVE MEDICINE, v. 12, NO 3, Page. 181-187 | en_US |
dc.identifier.issn | 1738-2696 | - |
dc.identifier.uri | http://link.springer.com/article/10.1007/s13770-015-0113-4 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11754/25545 | - |
dc.description.abstract | In recent years, perivascular stem cells (PVCs) have gained increasing attention as a promising source for regenerative medicine due to their greater differentiation potential compared to multipotent mesenchymal stem cells (MSCs). It has been reported that growth differentiation factor-5 (GDF-5) is involved in regulating proliferation and chondrogenic differentiation of MSCs. In this study, we investigated the effect of GDF-5 on the proliferation and chondrogenic differentiation of PVCs isolated from human umbilical cords. The supplementation of PVC cultures with GDF-5 (100 ng/mL) significantly enhanced their proliferative rate and augmented the size of pellets in micromass pellet cultures for chondrogenic induction. Although similar expression levels of chondrogenic-related genes were observed in chondrogenic pellets treated with GDF-5 compared to the pellets without GDF-5 treatment, these results indicate that supplementation of GDF-5 is able to acquire more chondrocytes when starting with equal amount of PVCs. Our study suggests that GDF-5 is an effective agent for the enhancement of PVC proliferation, thereby achieving a higher number of chondrocytes that are applicable in therapeutic doses for cartilage regeneration. | en_US |
dc.description.sponsorship | This study was supported by a grant of the Korea Health Technology R&D Project, Ministry of Health & Welfare, Republic of Korea (A120262). This research was also supported by The Health Fellowship Foundation. | en_US |
dc.language.iso | en | en_US |
dc.publisher | KOREAN TISSUE ENGINEERING REGENERATIVE MEDICINE SOC | en_US |
dc.subject | Perivascular stem cells | en_US |
dc.subject | Growth differentiation factor-5 | en_US |
dc.subject | Proliferation | en_US |
dc.subject | Chondrocytes | en_US |
dc.subject | AUTOLOGOUS CHONDROCYTE IMPLANTATION | en_US |
dc.subject | CARTILAGE REPAIR | en_US |
dc.subject | ARTICULAR CHONDROCYTES | en_US |
dc.subject | STROMAL CELLS | en_US |
dc.subject | BONE-MARROW | en_US |
dc.subject | IN-VITRO | en_US |
dc.subject | TISSUE | en_US |
dc.subject | EXPRESSION | en_US |
dc.subject | CULTURE | en_US |
dc.subject | GDF-5 | en_US |
dc.title | Supplementation of Growth Differentiation Factor-5 Increases Proliferation and Size of Chondrogenic Pellets of Human Umbilical Cord-Derived Perivascular Stem Cells | en_US |
dc.type | Article | en_US |
dc.relation.no | 3 | - |
dc.relation.volume | 12 | - |
dc.identifier.doi | 10.1007/s13770-015-0113-4 | - |
dc.relation.page | 181-187 | - |
dc.relation.journal | TISSUE ENGINEERING AND REGENERATIVE MEDICINE | - |
dc.contributor.googleauthor | An, Borim | - |
dc.contributor.googleauthor | Heo, Hye-Ryeon | - |
dc.contributor.googleauthor | Lee, Sungeun | - |
dc.contributor.googleauthor | Park, Jeong-Ah | - |
dc.contributor.googleauthor | Kim, Kye-Seong | - |
dc.contributor.googleauthor | Yang, Jeonghee | - |
dc.contributor.googleauthor | Hong, Seok-Ho | - |
dc.relation.code | 2015009287 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF MEDICINE[S] | - |
dc.sector.department | DEPARTMENT OF MEDICINE | - |
dc.identifier.pid | ks66kim | - |
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