Comparison between mixed lysate antigen and alpha-actinin antigen in ELISA for serodiagnosis of trichomoniasis

Abstract

The aim of this study was to identify an antigen suitable for ELISA for serodiagnosis of Trichomonas vaginalis (T. vaginalis) infection. Mixed lysate antigen (Ag) from eight strains of T. vaginalis and recombinant a-actinin protein was compared. The sera of three groups were examined by ELISA: 73 women infected with trichomoniasis served as a positive control, 31 male volunteers as a negative control, and 424 women attending an outpatient health screening at Hanyang University Guri Hospital. Based on the cutoff optical density for each Ag obtained with a negative control, the serosensitivity of the mixed lysate Ag (79.5%) was significantly higher than that of the a-actinin (52.1%) in the 73 patients with trichomoniasis. The specificity using lysate Ag and alpha-actinin was 100% and 96.8%, respectively. On the other hand, the positivity rate in 424 outpatients was 39.2% and 11.8% with mixed lysate and alpha-actinin Ag, respectively. Taken together, mixed lysate Ag showed higher sensitivity and specificity than alpha-actinin. Therefore, mixed lysate may be a better Ag than alpha-actinin for ELISA for the diagnosis of trichomoniasis. (C) 2015 Elsevier Ireland Ltd. All rights reserved.