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DC-Based Immunotherapy Combined with Low-Dose Methotrexate Effective in the Treatment of Advanced CIA in Mice

Title
DC-Based Immunotherapy Combined with Low-Dose Methotrexate Effective in the Treatment of Advanced CIA in Mice
Author
이혜순
Keywords
COLLAGEN-INDUCED ARTHRITIS; TOLEROGENIC DENDRITIC CELLS; RHEUMATOID-ARTHRITIS; T-CELLS; IN-VITRO; NECROSIS FACTOR; EXPRESS IL-4; THERAPY; TOLERANCE; INDUCTION
Issue Date
2015-06
Publisher
HINDAWI PUBLISHING CORPORATION
Citation
JOURNAL OF IMMUNOLOGY RESEARCH, v. 2015, NO 834085, Page. 1-15
Abstract
We have previously demonstrated that semimature dendritic cell- (smDC-) based immunotherapy is effective for the treatment of collagen-induced arthritis (CIA) prior to disease onset. In the present study, we examined the efficacy of combination therapy with smDCs and methotrexate (MTX) in advanced CIA with a score of 2-3. Combination therapy with low-dose MTX and type II collagen- (CII-) pulsed smDCs (CII-smDCs) was more effective in inhibiting disease progression than high or low-dose MTX alone or a combination of high dose MTX and CII-smDCs. The effect of CII-smDCs alone was also comparable to the combination therapy. CD4(+)Foxp3(+) Treg populations and IL-10 secretion markedly increased, and CII-specific autoreactive T cells decreased in mice treated with CII-smDCs alone or in combination with MTX. Combination therapy reduced the secretion of interferon-gamma (IFN-gamma) and IL-17 with little influence on the IL-4 secretion in the mixed leukocyte reaction. These results imply that the combination therapy with low-dose MTX and smDCs is effective in controlling advanced CIA by enhancing Treg population and suppresses antigen-specific Th1/Th17 immunity, rather than initiating Th1 to Th2 immune deviation. Our findings provide a better understanding of the DC therapy in combination with MTX for the treatment of patients with rheumatoid arthritis (RA).
URI
https://www.hindawi.com/journals/jir/2015/834085/http://hdl.handle.net/20.500.11754/25179
ISSN
2314-8861; 2314-7156
DOI
10.1155/2015/834085
Appears in Collections:
COLLEGE OF MEDICINE[S](의과대학) > MEDICINE(의학과) > Articles
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