322 143

Fluorescent and bioluminescent nanoprobes for in vitro and in vivo detection of matrix metalloproteinase activity

Title
Fluorescent and bioluminescent nanoprobes for in vitro and in vivo detection of matrix metalloproteinase activity
Author
김영필
Keywords
ECM; Enzyme activity; Imaging; Matrix metalloproteinase; Nanoprobe
Issue Date
2015-06
Publisher
KOREAN SOCIETY BIOCHEMISTRY & MOLECULAR BIOLOGY
Citation
BMB REPORTS, v. 48, NO 6, Page. 313-318
Abstract
Matrix metalloproteinases (MMPs) are zinc-dependent endopeptidases that degrade the extracellular matrix (ECM) and regulate the extracellular microenvironment. Despite the significant role that MMP activity plays in cell-cell and cell-ECM interactions, migration, and differentiation, analyses of MMPs in vitro and in vivo have relied upon their abundance using conventional immunoassays, rather than their enzymatic activities. To resolve this issue, diverse nanoprobes have emerged and proven useful as effective activity-based detection tools. Here, we review the recent advances in luminescent nanoprobes and their applications in in vitro diagnosis and in vivo imaging of MMP activity. Nanoprobes with the purpose of sensing MMP activity consist of recognition and detection units, which include MMP-specific substrates and luminescent (fluorescent or bioluminescent) nanoparticles, respectively. With further research into improvement of the optical performance, it is anticipated that luminescent nanoprobes will have great potential for the study of the functional roles of proteases in cancer biology and nanomedicine.
URI
http://www.bmbreports.org/journal/view.html?uid=163&vmd=Fullhttp://hdl.handle.net/20.500.11754/25057
ISSN
1976-6696; 1976-670X
DOI
10.5483/BMBRep.2015.48.6.054
Appears in Collections:
COLLEGE OF NATURAL SCIENCES[S](자연과학대학) > LIFE SCIENCE(생명과학과) > Articles
Files in This Item:
Fluorescent and bioluminescent nanoprobes for in vitro and in vivo detection of matrix metalloproteinase activity.pdfDownload
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE