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dc.contributor.author이민형-
dc.date.accessioned2017-01-10T00:31:50Z-
dc.date.available2017-01-10T00:31:50Z-
dc.date.issued2015-05-
dc.identifier.citationJOURNAL OF DRUG TARGETING, v. 23, NO 4, Page. 360-370en_US
dc.identifier.issn1061-186X-
dc.identifier.issn1029-2330-
dc.identifier.urihttp://www.tandfonline.com/doi/full/10.3109/1061186X.2014.1000336-
dc.identifier.urihttp://hdl.handle.net/20.500.11754/24998-
dc.description.abstractInhibition of microRNA-21 (miR-21) has been shown to promote apoptosis of cancer cells and to reduce tumor size in glioblastoma. However, efficient carriers for antisense-oligodeoxynucleotide (antisense-ODN) against miR-21 have not yet been developed. In this study, the R3V6 peptide (R3V6) was evaluated as a carrier of antisense-ODN. In a gel retardation assay, R3V6 formed a complex with an antisense-ODN. The serum stability assay showed that R3V6 protected it from nucleases more efficiently than polyethylenimine (PEI; 25 kDa, PEI25k). A Renilla luciferase gene with a 3'-untranslated region (3'-UTR) recognizable by miR-21 (psiCHECK2-miR-21-UTR) was constructed for the antisense-ODN assay. psiCHECK2-miR-21-UTR expressed less Renilla luciferase in the cells with a higher level of miR-21 due to the effect of miR-21. In an in vitro transfection assay, the R3V6 peptide delivered anti-miR-21 antisense-ODN into cells more efficiently than PEI (25 kDa, PEI25k) and lipofectamine. As a result, antisense-ODN/R3V6 complex inhibited miR-21 and increased Renilla luciferase expression more efficiently than antisense-ODN/PEI25k or antisense-ODN/Lipofectamine complexes in both C6 and A172 glioblastoma cells. Furthermore, the antisense-ODN/R3V6 complexes reduced the level of miR-21 and induced apoptosis of glioblastoma cells. These results suggest that the R3V6 peptide may be a useful carrier of antisense-ODN for glioblastoma gene therapy.en_US
dc.description.sponsorshipThis work was financially supported by the grant from the National Research Foundation of Korea, funded by the Ministry of Science, ICT and Future Planning (Grant number, NRF-2013R1A1A2059236) and the grant from Ministry of Health and welfare in Korea (Grant number, HI12C1210). The authors report no declarations of interest.en_US
dc.language.isoenen_US
dc.publisherINFORMA HEALTHCAREen_US
dc.subjectAmphiphilic peptideen_US
dc.subjectantisense-oligonucleotideen_US
dc.subjectgene therapyen_US
dc.subjectglioblastomaen_US
dc.subjectmicroRNA-21en_US
dc.titleDelivery of anti-microRNA-21 antisense-oligodeoxynucleotide using amphiphilic peptides for glioblastoma gene therapyen_US
dc.typeArticleen_US
dc.relation.no4-
dc.relation.volume23-
dc.identifier.doi10.3109/1061186X.2014.1000336-
dc.relation.page360-370-
dc.relation.journalJOURNAL OF DRUG TARGETING-
dc.contributor.googleauthorSong, Hojung-
dc.contributor.googleauthorOh, Binna-
dc.contributor.googleauthorChoi, Manbok-
dc.contributor.googleauthorOh, Jungju-
dc.contributor.googleauthorLee, Minhyung-
dc.relation.code2015002420-
dc.sector.campusS-
dc.sector.daehakCOLLEGE OF ENGINEERING[S]-
dc.sector.departmentDEPARTMENT OF BIOENGINEERING-
dc.identifier.pidminhyung-
Appears in Collections:
COLLEGE OF ENGINEERING[S](공과대학) > BIOENGINEERING(생명공학과) > Articles
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