Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이민형 | - |
dc.date.accessioned | 2017-01-10T00:31:50Z | - |
dc.date.available | 2017-01-10T00:31:50Z | - |
dc.date.issued | 2015-05 | - |
dc.identifier.citation | JOURNAL OF DRUG TARGETING, v. 23, NO 4, Page. 360-370 | en_US |
dc.identifier.issn | 1061-186X | - |
dc.identifier.issn | 1029-2330 | - |
dc.identifier.uri | http://www.tandfonline.com/doi/full/10.3109/1061186X.2014.1000336 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11754/24998 | - |
dc.description.abstract | Inhibition of microRNA-21 (miR-21) has been shown to promote apoptosis of cancer cells and to reduce tumor size in glioblastoma. However, efficient carriers for antisense-oligodeoxynucleotide (antisense-ODN) against miR-21 have not yet been developed. In this study, the R3V6 peptide (R3V6) was evaluated as a carrier of antisense-ODN. In a gel retardation assay, R3V6 formed a complex with an antisense-ODN. The serum stability assay showed that R3V6 protected it from nucleases more efficiently than polyethylenimine (PEI; 25 kDa, PEI25k). A Renilla luciferase gene with a 3'-untranslated region (3'-UTR) recognizable by miR-21 (psiCHECK2-miR-21-UTR) was constructed for the antisense-ODN assay. psiCHECK2-miR-21-UTR expressed less Renilla luciferase in the cells with a higher level of miR-21 due to the effect of miR-21. In an in vitro transfection assay, the R3V6 peptide delivered anti-miR-21 antisense-ODN into cells more efficiently than PEI (25 kDa, PEI25k) and lipofectamine. As a result, antisense-ODN/R3V6 complex inhibited miR-21 and increased Renilla luciferase expression more efficiently than antisense-ODN/PEI25k or antisense-ODN/Lipofectamine complexes in both C6 and A172 glioblastoma cells. Furthermore, the antisense-ODN/R3V6 complexes reduced the level of miR-21 and induced apoptosis of glioblastoma cells. These results suggest that the R3V6 peptide may be a useful carrier of antisense-ODN for glioblastoma gene therapy. | en_US |
dc.description.sponsorship | This work was financially supported by the grant from the National Research Foundation of Korea, funded by the Ministry of Science, ICT and Future Planning (Grant number, NRF-2013R1A1A2059236) and the grant from Ministry of Health and welfare in Korea (Grant number, HI12C1210). The authors report no declarations of interest. | en_US |
dc.language.iso | en | en_US |
dc.publisher | INFORMA HEALTHCARE | en_US |
dc.subject | Amphiphilic peptide | en_US |
dc.subject | antisense-oligonucleotide | en_US |
dc.subject | gene therapy | en_US |
dc.subject | glioblastoma | en_US |
dc.subject | microRNA-21 | en_US |
dc.title | Delivery of anti-microRNA-21 antisense-oligodeoxynucleotide using amphiphilic peptides for glioblastoma gene therapy | en_US |
dc.type | Article | en_US |
dc.relation.no | 4 | - |
dc.relation.volume | 23 | - |
dc.identifier.doi | 10.3109/1061186X.2014.1000336 | - |
dc.relation.page | 360-370 | - |
dc.relation.journal | JOURNAL OF DRUG TARGETING | - |
dc.contributor.googleauthor | Song, Hojung | - |
dc.contributor.googleauthor | Oh, Binna | - |
dc.contributor.googleauthor | Choi, Manbok | - |
dc.contributor.googleauthor | Oh, Jungju | - |
dc.contributor.googleauthor | Lee, Minhyung | - |
dc.relation.code | 2015002420 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF ENGINEERING[S] | - |
dc.sector.department | DEPARTMENT OF BIOENGINEERING | - |
dc.identifier.pid | minhyung | - |
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